Different degradation pathways for heterologous glycoproteins in yeast

FEBS Letters
H Holkeri, M Makarow

Abstract

Rat nerve growth factor receptor ectodomain (NGFRe) and Escherichia coli beta-lactamase were translocated into the yeast endoplasmic reticulum (ER), glycosylated, misfolded and rapidly degraded. NGFRe underwent ATP-dependent thermosensitive degradation independently of vesicular transport. Since no evidence for degradation by the cytoplasmic 26S proteosome complex could be obtained, NGFRe appeared to be degraded in the ER. Beta-lactamase exited the ER by vesicular traffic and was transported from the Golgi via the Vps10 receptor pathway to the vacuole for degradation. Machineries in the ER and the Golgi appear to recognize distinct structural features on misfolded heterologous proteins and guide them to different degradation pathways.

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Citations

Feb 9, 2010·Applied Microbiology and Biotechnology·Alimjan IdirisKaoru Takegawa
Feb 22, 2012·Applied Microbiology and Biotechnology·Martin PfefferDiethard Mattanovich
Jun 24, 2005·Bioscience, Biotechnology, and Biochemistry·Hisashi HoshidaRinji Akada
Mar 16, 2007·Progress in Retinal and Eye Research·Kunal RayMainak Sengupta
Mar 12, 2005·Pigment Cell Research·Andrea M Hall, Seth J Orlow
Oct 9, 2002·BMC Molecular Biology·Michael O AgaphonovMichael D Ter-Avanesyan
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Kengo SuyamaTakahiro Shintani
Jun 10, 2016·Archives of Dermatological Research·Yong Hyun KimJae Sung Hwang
Apr 14, 2004·The Journal of Cell Biology·Shilpa Vashist, Davis T W Ng
Dec 8, 2005·Biotechnology and Bioengineering·Andy Rakestraw, K Dane Wittrup
Sep 21, 2019·The Journal of Cell Biology·Zhihao Sun, Jeffrey L Brodsky
Oct 4, 2019·Biotechnology Progress·Shiyao Wang, Yong Ku Cho
Jan 19, 2020·Protein Expression and Purification·Uriel Rojas-SánchezMiguel A Gómez-Lim
Jul 2, 2021·Microbial Cell Factories·Aravind MadhavanParameswaran Binod
Feb 1, 2005·European Journal of Cell Biology·Anton Schmitz, Volker Herzog

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