Different detectability of high-risk HPV in smears from incident and prevalent high-grade squamous intraepithelial lesions of the cervix

Gynecologic Oncology
A SchneiderI Nindl

Abstract

Human papillomavirus (HPV) status in cervical smears from cervical intraepithelial neoplasia (CIN) 2/3 diagnosed in 36 of 892 women with a history of normal cytology and colposcopy (incident CIN 2/3) was compared with CIN 2/3 in 40 patients with a history of abnormal cytology (prevalent CIN 2/3). In all patients cervical smears for HPV testing and cytology and two cervigrams were taken. The scrapes were collected in hybrid capture assay solution and analyzed with the hybrid capture and general primer/type-specific primer polymerase chain reaction system (GP/TS-PCR) after DNA extraction. Patients with incident and prevalent CIN 2/3 were similar with respect to age. By GP/TS-PCR carried out under suboptimal conditions due to DNA extraction, HPV DNA was detected in 69.4% (25 of 36) of smears from incident CIN 2/3 compared to 95% (38 of 40) in prevalent CIN 2/3 (P = 0.003). Using hybrid capture, smears of incident CIN 2/3 were HPV positive in 50% (18 of 36) compared to 80% (32 of 40) in prevalent CIN 2/3 (P = 0.006). High-risk HPVs were significantly less common in smears from incident CIN 2/3 compared with prevalent CIN 2/3: 36.1% vs 72.5% by GP/TS-PCR (P = 0.001) and 47.2% vs 80% by hybrid capture assay (P = 0.003), respectively....Continue Reading

References

May 23, 1992·JAMA : the Journal of the American Medical Association·O LunguT C Wright
Jan 1, 1991·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·A SchneiderV Schneider
Jun 15, 1987·International Journal of Cancer. Journal International Du Cancer·A SchneiderL Gissmann
Sep 1, 1995·The Journal of Infectious Diseases·L F XiD A Galloway
Sep 20, 1995·Journal of the National Cancer Institute·G Y HoS Romney
Apr 1, 1995·American Journal of Obstetrics and Gynecology·K D HatchM W Abdel-Nour
Mar 1, 1995·Journal of Clinical Microbiology·M H SchiffmanM E Sherman
Jun 17, 1995·Lancet·J CuzickJ Guillebaud
Jul 1, 1994·Journal of Clinical Pathology·A FarthingK H Vousden
Mar 15, 1994·International Journal of Cancer. Journal International Du Cancer·A M de Roda HusmanP J Snijders
Oct 1, 1995·Journal of Clinical Microbiology·H L SmitsJ Ter Schegget

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Citations

Sep 27, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Yuping WuYing He
May 18, 1999·American Journal of Obstetrics and Gynecology·R H Kaufman, E Adam
Jun 1, 2014·Epidemiology and Infection·J TingJ S Smith
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Martin MobergUlf Gyllensten
Dec 13, 2002·International Journal of Cancer. Journal International Du Cancer·Nicolas F SchlechtEduardo L Franco
Dec 5, 2000·International Journal of Cancer. Journal International Du Cancer·A SchneiderM Dürst
Jun 11, 1999·International Journal of STD & AIDS·I Takac, B Gorisek

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