Different DNA polymerases are involved in the short- and long-patch base excision repair in mammalian cells

Biochemistry
P FortiniE Dogliotti

Abstract

Mammalian cells possess two distinct pathways for completion of base excision repair (BER): the DNA polymerase beta (Pol beta)-dependent short-patch pathway (replacement of one nucleotide), which is the main route, and the long-patch pathway (resynthesis of 2-6 nucleotides), which is PCNA-dependent. To address the issue of how these two pathways share their role in BER the ability of Pol beta-defective mammalian cell extracts to repair a single abasic site constructed in a circular duplex plasmid molecule was tested in a standard in vitro repair reaction. Pol beta-deficient extracts were able to perform both BER pathways. However, in the case of the short-patch BER, the repair kinetics was significantly slower than with Pol beta-proficient extracts, while the efficiency of the long-patch synthesis was unaffected by the loss of Pol beta. The repair synthesis was fully dependent on PCNA for the replacement of long patches. These data give the first evidence that in cell extracts DNA polymerases other than Pol beta are specifically involved in the long-patch BER. These DNA polymerases are also able to perform short-patch BER in the absence of PCNA, although less efficiently than Pol beta. These findings lead to a novel model where...Continue Reading

References

Dec 15, 1994·The Biochemical Journal·G FrosinaE Dogliotti
Apr 19, 1996·The Journal of Biological Chemistry·G FrosinaE Dogliotti

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Citations

May 8, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K W Caldecott
May 2, 2000·Immunology Letters·M A PahlavaniA Richardson
Dec 5, 1998·Biochimica Et Biophysica Acta·T Tsurimoto
Sep 12, 2003·DNA Repair·Keith W Caldecott
Jan 18, 2002·Journal of Neurochemistry·N MoorePhilip K Liu
Apr 12, 2000·European Journal of Biochemistry·G Frosina
Oct 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Barbara van Loon, Ulrich Hübscher
Oct 19, 2010·The Journal of Biological Chemistry·Rajendra PrasadSamuel H Wilson
Jul 30, 2008·The Journal of Biological Chemistry·Muralidhar L HegdeSankar Mitra
Jan 16, 2010·Journal of Toxicology and Environmental Health. Part a·Mi-Young SonJung-Suk Sung
Apr 15, 2006·Mutagenesis·Katie K L ChanGrigory L Dianov
Aug 4, 2005·Nucleic Acids Research·Valeria SimonelliPaola Fortini
Oct 9, 2012·Nucleic Acids Research·Rajendra PrasadSamuel H Wilson
Jul 25, 2008·Biology of Reproduction·Diwi AllenChristi A Walter
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S H WilsonB J Vande Berg
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S E Tsutakawa, P K Cooper
Jun 29, 2000·Annual Review of Biochemistry·A K McCulloughR S Lloyd
Jun 11, 2004·Annual Review of Biochemistry·Yuan LiuRobert A Bambara
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·C J Norbury, I D Hickson
Jan 30, 2013·International Journal of Molecular Sciences·Claudia GiachinoValentina Turinetto
Feb 10, 2000·Archives of Biochemistry and Biophysics·S M Nielsen-Preiss, R L Low
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert W SobolSamuel H Wilson
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·C A ClairmontJ B Sweasy
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·M J LongleyW C Copeland
Nov 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wensheng WangRobert A Bambara
Jan 7, 2011·Journal of Nucleic Acids·Rajesh P RastogiRajeshwar P Sinha
Oct 30, 2014·The New England Journal of Medicine·Donal P McLornanGhulam J Mufti
Oct 29, 2010·Journal of Nucleic Acids·Rihito MoritaSeiki Kuramitsu

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