PMID: 9440318Jan 24, 1998Paper

Different DNA repair time courses in human lymphoid cells after UVA, UVA1, UVB and PUVA in vitro

Journal of Photochemistry and Photobiology. B, Biology
O KleinauB Lanto

Abstract

The time course of DNA repair was investigated using the nucleoid sedimentation technique. Human lymphoid cells were irradiated with membrane related equitoxic doses of UVB, UVA/B, UVA and UVA1. Additionally, PUVA treatment of cells using two different light sources was performed. It was found that in the case of UVB and UVA/B irradiation of the cells, DNA repair activity arose lasting not longer than 4 h. However, in the case of UVA and UVA1 no repair activity was detectable with the used method but an increasing nucleoid density believed to indicate apoptosis. This could be shown with the same irradiation protocol. When the irradiation dose of UVA and UVA1 was lowered to one fifth of the equitoxic dose neither repair activity nor apoptosis was detected. There was no difference between UVA and UVA1 with respect to the nucleoid density time course. Hence, the initial absorbing chromophore is probably located in the UVA1 region. In the case of PUVA, we found DNA repair that was complete after 12 h. The UVB part in the irradiation spectrum of a PUVA system had an accelerating effect on the repair kinetics. The time course of DNA density appears to be related to subsequent cell behaviour: apoptosis or incomplete DNA repair includi...Continue Reading

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Citations

Nov 18, 2000·Journal of Photochemistry and Photobiology. B, Biology·A RappK O Greulich
Nov 18, 2000·Journal of Photochemistry and Photobiology. B, Biology·H M SaloH T Penttilä
Dec 24, 2013·Journal of Photochemistry and Photobiology. B, Biology·Emanuelle B F SilvaJosé P Siqueira-Júnior
Feb 16, 2013·Veterinary and Comparative Oncology·H M Wilson-RoblesS Sheppard
Dec 25, 2002·The Tohoku Journal of Experimental Medicine·Zuhal HamurcuEkrem Aktas
Apr 26, 2002·Cell Death and Differentiation·A B SantamariaH N Ananthaswamy

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