Abstract
In immunological reactions, leukocytes need to travel from the intravascular space through the vessel wall into the surrounding tissue. The first step in this process is leukocyte rolling, which has often been studied in anesthetized animals. In this study, we investigated the effect of pentobarbital, Hypnorm and both components of the latter, fentanyl and fluanisone, on this primary leukocyte-endothelial cell interaction. Using intravital brightfield video microscopy, observations were made in postcapillary venules in the intact skin of the nailfold of trained conscious Lewis rats. Subsequently, the animals were anesthetized and observations were made in vivo. Leukocyte rolling was significantly elevated after injection of Hypnorm or fentanyl, while pentobarbital and fluanisone had no effect. None of the anesthetics affected leukocyte rolling velocity. Blood flow was significantly increased only after injection of Hypnorm and fluanisone. No correlation existed between the relative changes in leukocyte rolling and concomitant changes in blood flow. The results show that the level of leukocyte rolling can be affected by anesthetics. These changes are probably not mediated by changes in local hemodynamics. Pentobarbital anesthesi...Continue Reading
Citations
Dec 21, 2007·Archives of Orthopaedic and Trauma Surgery·Mark MelnykPeter Augat
Jun 16, 2000·Journal of Immunological Methods·M D BeckerJ T Rosenbaum
Aug 11, 2001·Journal of Neuroimmunology·S D HouseS L Chang
Aug 12, 2003·International Journal of Radiation Oncology, Biology, Physics·Meritxell MollàJulián Panés
Jan 15, 2000·Annals of Surgery·R J BeukM G oude Egbrink
Oct 29, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·W A Menth-Chiari
Feb 14, 2002·The Journal of Investigative Dermatology·Mirjam G A oude EgbrinkGeert Jan Tangelder
Mar 16, 2006·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Chol Kim, Atsuhiro Sakamoto
Feb 14, 1998·The Journal of Surgical Research·R J BeukM G oude Egbrink
Feb 23, 2008·Surgery·Richard S FlintJohn A Windsor
May 9, 2006·Shock·Zoë L S BrookesNicola J Brown
Nov 24, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K D SchaserT Mittlmeier
Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·M Michele MariscalcoC Wayne Smith