Different effects of calcium channel blockers on matrix metalloproteinase-2 expression in cultured rat cardiac fibroblasts

Journal of Cardiovascular Pharmacology
Hong YueJong-Dae Lee

Abstract

The cardiac effects of calcium channel blockers (CCBs) related to cardiac remodeling are inconsistent. Matrix metalloproteinases (MMPs) contribute to tissue remodeling. Cardiac fibroblasts play an important role in the regulation of collagen degradation by MMPs. Using gelatin zymography, Western blotting, Griess reagent, and a calcium kit-fluo 3, we investigated the effects of nifedipine, verapamil, diltiazem, and amlodipine on MMP-2 expression and further elucidate the mechanisms in cultured rat cardiac fibroblasts. Nifedipine increased and amlodipine decreased the expression of MMP-2; however, neither verapamil nor diltiazem altered MMP-2 expression. Nifedipine also increased nitrite production, and this increase was blunted by a nitric oxide (NO) synthases inhibitor (L-NAME). Nifedipine-induced MMP-2 expression was also blunted by L-NAME. An NO donor (sodium nitroprusside) induced MMP-2 expression. Data indicated that nifedipine might increase MMP-2 expression through a possible NO-dependent pathway. Amlodipine had no influence on nitrite production. The amlodipine-induced decrease of MMP-2 expression was abolished by two protein tyrosine kinase inhibitors, genistein and herbimycin A, indicating that amlodipine might decreas...Continue Reading

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Citations

Nov 9, 2010·Naunyn-Schmiedeberg's Archives of Pharmacology·Diogo M O MarçalJose E Tanus-Santos
Nov 15, 2007·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Graziano OnderFrancesco Landi
Oct 22, 2008·European Journal of Pharmacology·Marcio L L MartinezJose E Tanus-Santos
Jul 19, 2008·European Journal of Pharmacology·Marcio L L MartinezJose E Tanus-Santos
May 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Hanzhong LiuGary A Visner
Mar 27, 2007·Journal of Dental Research·P BullonH N Newman
Jan 21, 2006·Journal of Cardiovascular Pharmacology·Marcio L L MartinezJose E Tanus-Santos
Dec 14, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Manuela Martins-GreenMin Yao
Nov 5, 2019·Aging·Ji Min KimByung-Joo Lee
May 8, 2018·Free Radical Biology & Medicine·Wei LiW H Wilson Tang
Apr 25, 2019·Skin Pharmacology and Physiology·Ewa Klara StuermerAnna-Lena Severing

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