Different effects of lysophosphatidic acid receptor-2 (LPA2 ) and LPA5 on the regulation of chemoresistance in colon cancer cells

Journal of Receptor and Signal Transduction Research
Kaichi IshimotoToshifumi Tsujiuchi

Abstract

Lysophosphatidic acid (LPA) is a simple physiological lipid and exhibits several biological functions by binding to G-protein-coupled LPA receptors (LPA receptor-1 (LPA1) to LPA6). The present study aimed to evaluate whether LPA signaling via LPA2 and LPA5 is involved in the chemoresistance to anticancer drugs in colon cancer DLD1 cells. In cell survival assay, cells were treated with fluorouracil (5-FU) every 24 h for 2 days. The cell survival rate to 5-FU of DLD1 cells was significantly decreased by LPA treatment. In the presence of LPA, the cell survival rate to 5-FU was significantly elevated by LPA5 knockdown. Before initiation of the cell survival assay, cells were pretreated with an LPA2 agonist, GRI-977143. The cell survival rate to 5-FU was markedly increased in DLD1 cells treated with GRI-977143. In the presence of GRI-977143, the elevated cell survival rate of DLD1 cells was reduced by LPA2 knockdown. To assess the effects of LPA2 and LPA5 on the enhancement of chemoresistance, long-term 5-FU treated (DLD-5FU) cells were generated from DLD1 cells. The cell survival rate to 5-FU of DLD-5FU cells were significantly elevated by LPA5 knockdown. GRI-977143 treatment increased the cell survival rate to 5-FU of DLD-5FU cell...Continue Reading

References

Jul 17, 2001·British Journal of Pharmacology·S M HouraniD J Prentice
Jun 16, 2006·The Journal of Biological Chemistry·Chang-Wook LeeJerold Chun
Jan 18, 2007·The Oncologist·Richard M GoldbergCarol Viele
Mar 25, 2010·Prostaglandins & Other Lipid Mediators·Mu-En LinJerold Chun
Oct 5, 2010·Virchows Archiv : an International Journal of Pathology·Megumu TsujinoToshifumi Tsujiuchi
Oct 18, 2012·Annual Review of Pharmacology and Toxicology·Satish CheepalaJohn D Schuetz
Mar 20, 2014·Journal of Lipid Research·Yun C YungJerold Chun
Dec 17, 2014·Journal of Biochemistry·Shizu AikawaJunken Aoki
Jan 17, 2015·Biomolecules & Therapeutics·Nicole C Stoddard, Jerold Chun
Dec 21, 2016·Biochemical and Biophysical Research Communications·Kaede TakahashiToshifumi Tsujiuchi
Jan 9, 2018·Biochemical and Biophysical Research Communications·Kaori FukushimaToshifumi Tsujiuchi
Jan 26, 2018·Journal of Receptor and Signal Transduction Research·Kaede TakahashiToshifumi Tsujiuchi
Jun 3, 2018·Experimental Cell Research·Kaede TakahashiToshifumi Tsujiuchi
Aug 11, 2018·Biochemical and Biophysical Research Communications·Kaede TakahashiToshifumi Tsujiuchi
Aug 1, 2019·Biochemical and Biophysical Research Communications·Kanako MinamiToshifumi Tsujiuchi

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.