Different effects of Tetrahymena IFT172 domains on anterograde and retrograde intraflagellar transport

Molecular Biology of the Cell
Che-Chia Tsao, Martin A Gorovsky

Abstract

Intraflagellar transport (IFT) particles are multiprotein complexes that move bidirectionally along the cilium/flagellum. The Tetrahymena IFT172 gene encodes a protein with an N-terminal WD domain (WDD) and a C-terminal repeat domain (RPD). Epitope-tagged Ift172p localized to the basal body and in cilia along the axoneme, and IFT172 knockout cells lost cilia and motility. Using serial deletion constructs to rescue the knockout cells, we found that neither the WDD nor the RPD alone is sufficient to assemble cilia. Ift172p containing only the WDD or the RPD failed to enter cilia. Constructs with a partial truncation of the RPD still rescued although cilia were assembled less efficiently, indicating that the WDD and a part of the RPD are sufficient for anterograde transport. Partial truncation of the RPD caused the accumulation of truncated Ift172p itself and of Ift88p at ciliary tips, suggesting that IFT turnaround or retrograde transport was affected. These results implicate different regions of Ift172p in different steps of the IFT process.

References

Jan 1, 1975·Methods in Cell Biology·M A GorovskyG L Pleger
Dec 1, 1992·The Journal of Cell Biology·K A Johnson, J L Rosenbaum
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M C Yao, C H Yao
Oct 1, 1986·Developmental Biology·L A PerkinsJ G Culotti
Feb 1, 1969·The Journal of Cell Biology·J L Rosenbaum, K Carlson
Jan 1, 1982·Methods in Cell Biology·L Van De WaterJ B Olmsted
Dec 11, 1994·Nucleic Acids Research·J GaertigM A Gorovsky
Jul 22, 1993·Nature
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K G KozminskiJ L Rosenbaum
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchultzC P Ponting
Feb 11, 1999·The Journal of Cell Biology·G J PazourG B Witman
May 14, 1999·Trends in Biochemical Sciences·T F SmithE J Neer
Sep 30, 1999·Methods in Cell Biology·J Frankel
Sep 30, 1999·Methods in Cell Biology·K R Stuart, E S Cole
May 2, 2000·The Journal of Biological Chemistry·P W Howard, R A Maurer
Jun 10, 2000·The Journal of Eukaryotic Microbiology·J Gaertig
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yuhua ShangMartin A Gorovsky
Mar 28, 2002·The Journal of Cell Biology·Gregory J PazourJoseph C Besharse
Nov 5, 2002·Nature Reviews. Molecular Cell Biology·Joel L Rosenbaum, George B Witman
Mar 26, 2003·Experimental Cell Research·Courtney J HaycraftBradley K Yoder
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Jonathan M Scholey

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Citations

Mar 24, 2011·Nature Reviews. Molecular Cell Biology·Hiroaki Ishikawa, Wallace F Marshall
Nov 21, 2008·Molecular Biology of the Cell·Vidyalakshmi RajagopalanDavid J Asai
Mar 20, 2009·Journal of Cell Science·Limin Hao, Jonathan M Scholey
Oct 25, 2008·PLoS Genetics·James J CollinsKerry Kornfeld
Dec 30, 2008·PLoS Genetics·John A FollitGregory J Pazour
Sep 1, 2009·PloS One·Polloneal Jymmiel R OcbinaKathryn V Anderson
Jul 8, 2009·Eukaryotic Cell·Drashti DaveJacek Gaertig
Jan 15, 2016·Journal of Human Genetics·Elise SchaeferHélène Dollfus
Nov 29, 2011·Differentiation; Research in Biological Diversity·Michael TaschnerEsben Lorentzen
May 20, 2008·Science in China. Series C, Life Sciences·Junmin Pan
Jun 30, 2009·Cell Motility and the Cytoskeleton·David J AsaiDavid E Wilkes
Mar 3, 2009·Cell Motility and the Cytoskeleton·John A FollitGregory J Pazour
Jul 7, 2011·Biology of the Cell·Cathy Fisch, Pascale Dupuis-Williams
Jul 22, 2014·Traffic·Kayalvizhi Madhivanan, Ruben Claudio Aguilar
Feb 20, 2015·Molecular Biology of the Cell·Paulina UrbanskaDorota Wloga
Oct 22, 2013·American Journal of Human Genetics·Jan HalbritterFriedhelm Hildebrandt
Jun 13, 2018·The FEBS Journal·Stine Kjaer MorthorstLotte Bang Pedersen
Nov 23, 2018·Essays in Biochemistry·Jacob T CroftJohanna L Höög
Oct 3, 2019·Proceedings. Biological Sciences·Eduardo MorenoRalf J Sommer
Aug 2, 2018·Biochemical Society Transactions·Anthony J Roberts
Nov 10, 2018·Nature Communications·Qianmin WangNaoko Mizuno
Mar 20, 2019·International Journal of Molecular Sciences·Lucilla FabbriNathalie M Mazure
Apr 2, 2020·Journal of Cell Science·Laura VuoloDavid J Stephens
Jun 30, 2016·Cold Spring Harbor Perspectives in Biology·Michael Taschner, Esben Lorentzen
Nov 6, 2009·Science Signaling·Amir M AshiqueAndrew S Peterson

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