Different effects of transcriptional regulators MarA, SoxS and Rob on susceptibility of Escherichia coli to cationic antimicrobial peptides (CAMPs): Rob-dependent CAMP induction of the marRAB operon.

Microbiology
Douglas M Warner, Stuart B Levy

Abstract

Cationic antimicrobial peptides (CAMPs), a component of the mammalian immune system, protect the host from bacterial infections. The roles of the Escherichia coli transcriptional regulators MarA, SoxS and Rob in susceptibility to these peptides were examined. Overexpression of marA, either in an antibiotic-resistant marR mutant or from a plasmid, decreased bacterial susceptibility to CAMPs. Overexpression of the soxS gene from a plasmid, which decreased susceptibility to antibiotics, unexpectedly caused no decrease in CAMP susceptibility; instead it produced increased susceptibility to different CAMPs. Deletion or overexpression of rob had little effect on CAMP susceptibility. The marRAB operon was upregulated when E. coli was incubated in sublethal amounts of CAMPs polymyxin B, LL-37 or human beta-defensin-1; however, this upregulation required Rob. Deletion of acrAB increased bacterial susceptibility to polymyxin B, LL-37 and human beta-defensin-1 peptides. Deletion of tolC yielded an even greater increase in susceptibility to these peptides and also led to increased susceptibility to human alpha-defensin-2. Inhibition of cellular proton-motive force increased peptide susceptibility for wild-type and acrAB deletion strains; h...Continue Reading

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Citations

Nov 16, 2011·Antimicrobial Agents and Chemotherapy·Ida M ListerStuart B Levy
Sep 29, 2011·Applied and Environmental Microbiology·Rebecca M LennenBrian F Pfleger
Sep 25, 2010·PLoS Pathogens·Jaroslaw ZdziarskiUlrich Dobrindt
Sep 18, 2013·The Journal of Antimicrobial Chemotherapy·Cristian Ruiz, Stuart B Levy
May 25, 2013·Future Microbiology·Tony VelkovJian Li
Jun 27, 2015·Frontiers in Microbiology·João AnesMarta Martins
Feb 11, 2015·BioMed Research International·Zhiliang YuJuanping Qiu
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Aug 16, 2016·International Journal of Antimicrobial Agents·Sophie BaronAbiola Olumuyiwa Olaitan
Aug 10, 2016·Cold Spring Harbor Perspectives in Medicine·Michael J TrimbleRobert E W Hancock
May 2, 2021·Veterinary Microbiology·Lin LiFeifei Sun

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