Different effects on reporter gene expression by distinct L1-ORF2 segements

Yi chuan = Hereditas
Xiao-Cui DuanZhan-Jun Lu

Abstract

An intact L1 define element is 6 kb in length in human genome. The majority of the L1s is truncated and has direction difference, implying that it is interesting to study the effects of different length and directions of L1s on gene. In this work, 7 different segments were obtained from L1-open reading frame 2 (ORF2), each of which was 280 bp in length. Each segment was connected into 8 repeats in head and tail tandem manner and was inserted to downstream of GFP gene in different directions in pEGFP-C1. The inserted ORF2 segments in the sense orientation caused much stronger inhibition on gene transcription and protein expression than antisense sequences did. Among all segments, the first and ninth 280 bp segments of ORF2 in both orientations induced weaker inhibition on gene transcription than other segments in the same orientations and did not induce transcriptional elongation. The distribution of Alu in most regions of genome was inverse ratio with L1. The inserted Alus in both orientations inhibited GFP gene expression, but the inhibition in antisense orientation was stronger than that in sense orientation and the sense Alu was the sequence of inducing transcription elongation. A-rich of ORF2 was probably the molecular basi...Continue Reading

References

Nov 30, 2000·Nucleic Acids Research·C AlemánP L Deininger
Aug 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elena AllenYork Marahrens
Mar 18, 2005·Nature·Mark T RossDavid R Bentley
Mar 24, 2006·Nucleic Acids Research·Victoria P BelancioPrescott Deininger
May 10, 2006·Nucleic Acids Research·Julien Häsler, Katharina Strub
Sep 5, 2006·PLoS Computational Biology·Zhong WangTerrence S Furey
Oct 6, 2006·Nucleic Acids Research·Julien Häsler, Katharina Strub

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Citations

Jul 3, 2010·Science China. Life Sciences·Kang YinZhanjun Lü

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