PMID: 8601323Apr 1, 1996Paper

Different efficacy of various blocking reagents to eliminate interferences by human antimouse antibodies with a two-site immunoassay

Clinical Biochemistry
J Reinsberg

Abstract

The efficacy of three reagents to eliminate interferences with the cancer antigen 125 (CA-125) assay Enzymun CA-125 II by human antimouse antibodies (HAMA) formed by patients after injection of the murine anti-CA-125 antibody OC125 is compared. Apparent CA-125 concentrations of 14 serum samples obtained from 6 patients after multiple injections of 1 mg radiolabeled OC125 F(ab')2 fragments were measured with the Enzymun CA-125 II before and after preincubation, either with nonspecific mouse IgG, with the polymerized mouse IgG MAK-33, or with the commercially available HAMA-blocking reagent IIR. In all samples with HAMA concentrations ranging from 341 to 46900 microg/L, false-positive CA-125 values were measured with the Enzymun CA-125 II, which could be reduced by preincubation with the HAMA-blocking reagents. However, although after preincubation with 2 g/L IIR for all samples the CA-125 concentrations measured were reduced to values within the normal range, after preincubation with 0.7 g/L of polyclonal mouse IgG for five samples and after preincubation with 0.7 g/L of MAK-33 for all samples also the reduced values were considerably elevated. Larger amounts of mouse IgG or MAK-33 led only to a slight reduction of the remaining...Continue Reading

References

Jan 1, 1989·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·J C ReynoldsS M Larson
Jan 1, 1988·International Journal of Cancer. Supplement = Journal International Du Cancer. Supplement·G SchulzK Bosslet
May 1, 1993·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·J Reinsberg, W Nocke

❮ Previous
Next ❯

Citations

Mar 28, 2008·Veterinary Clinical Pathology·Philip F SolterD David Sisson
Apr 25, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·S C KazmierczakK P Briley
Feb 10, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Agnès GeorgesJean-Benoît Corcuff
Jun 16, 2011·International Journal of Molecular Sciences·Hongtao ZhangXiaowei Xu
Oct 6, 1999·European Journal of Obstetrics, Gynecology, and Reproductive Biology·N P KoperM J Crooy
Apr 24, 2012·Biochimica Et Biophysica Acta·Lian LamHongtao Zhang
Aug 4, 2007·Endocrinology and Metabolism Clinics of North America·D Robert Dufour
May 26, 2004·Clinics in Laboratory Medicine·George G Klee
Oct 13, 2017·Journal of Endocrinological Investigation·D M Nazato, J Abucham
Mar 2, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Alexandre PerrierMathieu Boissan
Jul 6, 2010·Clinical Chemistry·J Alan EricksonEdward R Ashwood
Jun 3, 2000·Archives of Pathology & Laboratory Medicine·G G Klee
Jun 23, 2009·Journal of Immunological Methods·Andrew N Hoofnagle, Mark H Wener

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.