Different efficiencies of the Tag and AlkA DNA glycosylases from Escherichia coli in the removal of 3-methyladenine from single-stranded DNA

FEBS Letters
S Bjelland, E Seeberg

Abstract

Escherichia coli possesses two different DNA repair glycosylases, Tag and AlkA, which have similar ability to remove the alkylation product 3-methyladenine from double-stranded DNA. In this study we show that these enzymes have quite different activities for the excision of 3-methyladenine from single-stranded DNA, AlkA being 10-20 times more efficient than Tag. We propose that AlkA and perhaps other glycosylases as well may have an important role in the excision of base damage from single-stranded regions transiently formed in DNA during transcription and replication.

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Citations

Oct 31, 2009·Carcinogenesis·Nidhi ShrivastavJohn M Essigmann
Dec 18, 2007·Journal of Bacteriology·Raquel Paes da RochaRodrigo S Galhardo
Sep 24, 2004·DNA Repair·Finn DrabløsHans E Krokan
Aug 30, 2008·Critical Reviews in Biochemistry and Molecular Biology·Joke Baute, Anne Depicker
Jul 20, 2019·The Journal of Biological Chemistry·Suzanne J AdmiraalPatrick J O'Brien
Aug 9, 2020·Malaria Journal·Nattapon PinthongPorntip Chavalitshewinkoon-Petmitr
Sep 29, 2006·The Journal of Physical Chemistry. B·Lesley R RutledgeStacey D Wetmore
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Feb 19, 2002·Chemical Reviews·Sheila S. David, Scott D. Williams

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