PMID: 9449240Aug 1, 1997Paper

Different estrogen receptor structural domains are required for estrogen- and tamoxifen-dependent anti-proliferative activity in human mammary epithelial cells expressing an exogenous estrogen receptor

The Journal of Steroid Biochemistry and Molecular Biology
D A ZajchowskiM Bartholdi

Abstract

Estrogen (E) inhibits the growth of both non-tumorigenic, immortal human mammary epithelial cells (HMEC) and breast cancer cells which stably express exogenous estrogen receptors (ER). The anti-estrogenic compounds 4-hydroxy-tamoxifen (HT) and ICI 164384 (ICI) have different effects on the growth of the ER-transfectants. HT is a potent growth inhibitor, while ICI has no effect by itself but is able to block the anti-proliferative effects of E and HT. In order to elucidate the mechanism by which E or HT-bound ER inhibit cell growth, we have evaluated the effects of these compounds on the growth of HMEC stably expressing ER with mutations or deletions in the N-terminal A/B domain, the DNA-binding domain (DBD), and the C-terminal ligand-binding domain. These studies revealed that E and HT require different structural domains of the ER for their anti-proliferative activities. The N-terminal A/B domain is required for HT-, but not E-dependent growth inhibition. The DNA-binding domain of the ER is not essential for HT-mediated anti-proliferative effects, but is important for E-dependent activity. The effect of ER mutations on the ligand-inducible expression of the endogenous progesterone receptor (PR) and pS2 genes was also evaluated...Continue Reading

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M GarciaH Rochefort
Feb 1, 1992·Molecular Endocrinology·P WebbP J Kushner
Jan 1, 1991·Annual Review of Genetics·H Gronemeyer
Jul 1, 1990·Molecular Endocrinology·D A Nielsen, D J Shapiro
Nov 1, 1988·Trends in Genetics : TIG·S Green, P Chambon
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·V Band, R Sager
Dec 24, 1987·Cell·V KumarP Chambon
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M R Stampfer, J C Bartley
Jan 1, 1984·Journal of Steroid Biochemistry·G L GreeneE V Jensen
Jan 1, 1984·Pharmacology & Therapeutics·B J Furr, V C Jordan
Mar 1, 1995·Molecular and Cellular Biology·Y SadovskyP J Kushner
Jun 11, 1993·Annals of the New York Academy of Sciences·M G ParkerR White
Mar 1, 1996·Molecular Carcinogenesis·T K ChenP H Brown

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Citations

Apr 2, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ming Zhao ChengLance E Lanyon
Dec 8, 2004·Endocrine Reviews·Matthew H Herynk, Suzanne A W Fuqua

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