Different expression patterns of growth factors in rat fetuses with spina bifida aperta after in utero mesenchymal stromal cell transplantation

Cytotherapy
Hui LiZhengwei Yuan

Abstract

In a previous study, we successfully devised a prenatal surgical approach and transplanted mesenchymal stromal cells (MSCs) to fetal rat spinal column to treat retinoic acid-induced neural tube defects in rat. Our results show that MSCs survived, migrated and differentiated into neural lineage cells. We intended to study various growth factor expressions in rat fetal spinal cords with spina bifida aperta after in utero MSC transplantation and the effect of in vivo growth factor introduction for prenatal spina bifida treatment. Pregnant rats were treated with retinoic acid on embryonic day 10 and then received fetal surgery for MSC transplantation and/or lentiviral epidermal growth factor (EGF) injection on embryonic day 16; various growth factor expression in spinal cords from embryonic day 20 fetuses were analyzed by means of quantitative reverse transcriptase-polymerase chain reaction. Terminal deoxynucleotidyl transferase dUTP nick end labeling analysis was performed to observe spinal tissue apoptosis. Growth factor expression was dysregulated in spinal cords with spina bifida. After MSC transplantation, we observed significantly increased expression of EGF, fibroblast growth factor (FGF)-8, FGF-2 and FGF-20 in the MSC trans...Continue Reading

References

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Citations

Jul 15, 2015·Birth Defects Research. Part A, Clinical and Molecular Teratology·Wei MaZhengwei Yuan
Jun 13, 2015·Human Gene Therapy·Jennifer L GoriDavid Bumcrot
Feb 13, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Priyadarsini KumarAijun Wang
Jun 10, 2017·Stem Cells International·Neeladri Chowdhury, Atsushi Asakura
Mar 21, 2021·Stem Cell Reviews and Reports·Alireza Soltani KhaboushanMasoumeh Majidi Zolbin
Jul 7, 2020·Current Research in Translational Medicine·A DugasL Guilbaud

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