Different fibroblast subpopulations of the eye: a therapeutic target to prevent postoperative fibrosis in glaucoma therapy

Experimental Eye Research
Thomas StahnkeRudolf Guthoff

Abstract

The aim of this study is the characterization of fibroblasts mainly responsible for fibrosis processes associated with trabeculectomy or microstent implantation for glaucoma therapy. Therefore we isolated human primary fibroblasts from choroidea, sclera, Tenon capsule, and orbital fat tissues. These fibroblast subpopulations were analysed in vitro for expression of the extracellular matrix components which are responsible for postoperative scarring in glaucoma therapy. For scarring the proteins of the collagen family are predominant and so we focused on the expression of collagen I, collagen III and collagen VI in every fibroblast subpopulation. Also, the extracellular matrix protein fibronectin which crosslinks collagen fibres or other extracellular matrix components and cell surfaces, was analyzed. Collagen I, III and VI were prominent in every fibroblast subpopulation. The highest amounts of collagen III were found in hCF and hOF, whereas the signal in hSF and hTF was negligible. Additionally, there is a link between scarring processes and proliferating potential of fibroblasts, in case of microstent implantation triggered through the infiltration of inflammatory cells. Thus we analyzed fibroblast subpopulations for the pres...Continue Reading

References

Sep 1, 1969·The British Journal of Ophthalmology·A C Molteno
Jan 1, 1993·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·A FujitaniS Noda
Jun 1, 1996·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·H MietzG K Krieglstein
Jan 20, 1999·The British Journal of Ophthalmology·K S LimP T Khaw
Oct 12, 2000·Eye·M F CordeiroP T Khaw
May 7, 2003·Circulation·J Eduardo SousaMarco A Costa
Aug 5, 2003·Journal of Glaucoma·Akif OzdamarMurat Karacorlu
Jun 22, 2005·Survey of Ophthalmology·C Robert BernardinoClaus H Dohlman
Jun 17, 2006·Journal of Glaucoma·Jens F JordanGünter K Krieglstein
Oct 24, 2006·American Journal of Ophthalmology·Steven J GeddeJoyce C Schiffman
Mar 4, 2008·Experimental Eye Research·Ted S Acott, Mary J Kelley
Jun 4, 2008·Ophthalmology·Don S MincklerKuldev Singh
Jun 10, 2008·American Journal of Ophthalmology·Jorge A AlvaradoLillian C Lee
Aug 7, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·T S DietleinG K Krieglstein
Dec 24, 2008·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Neville A McBrienAlex Gentle
Feb 3, 2009·Investigative Ophthalmology & Visual Science·Farnaz MemarzadehDean Eliott
Aug 15, 2009·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Christoph TappeinerBeatrice E Frueh
Oct 7, 2009·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·R F GuthoffK-P Schmitz
Mar 25, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Marian LöblerRudolf Guthoff
Jul 26, 2011·Current Eye Research·Reto AllemannOliver Stachs

❮ Previous
Next ❯

Citations

Jul 28, 2013·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·R AllemannR F Guthoff
Feb 5, 2015·Journal of Glaucoma·Ahmed Awadein, Yasmine M El Sayed
Jul 15, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Karsten VossMarina Hovakimyan
Nov 17, 2012·Current Opinion in Pharmacology·Alastair LockwoodPeng Tee Khaw
Feb 18, 2018·The Journal of Biological Chemistry·Alexander EckersleyMichael J Sherratt
Mar 26, 2016·The Open Ophthalmology Journal·Masoumeh B MasoumpourM Reza Razeghinejad
Oct 12, 2019·Journal of Orthopaedic Surgery and Research·Guirun XiongXiaolei Li
Nov 12, 2020·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Mario MontelongoWilliam Eric Sponsel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.