Different forms of glycine- and GABA(A)-receptor mediated inhibitory synaptic transmission in mouse superficial and deep dorsal horn neurons.

Molecular Pain
Wayne B AndersonRobert John Callister

Abstract

Neurons in superficial (SDH) and deep (DDH) laminae of the spinal cord dorsal horn receive sensory information from skin, muscle, joints and viscera. In both regions, glycine- (GlyR) and GABAA-receptors (GABAARs) contribute to fast synaptic inhibition. For rat, several types of GABAAR coexist in the two regions and each receptor type provides different contributions to inhibitory tone. Recent work in mouse has discovered an additional type of GlyR, (containing alpha 3 subunits) in the SDH. The contribution of differing forms of the GlyR to sensory processing in SDH and DDH is not understood. Here we compare fast inhibitory synaptic transmission in mouse (P17-37) SDH and DDH using patch-clamp electrophysiology in transverse spinal cord slices (L3-L5 segments, 23 degrees C). GlyR-mediated mIPSCs were detected in 74% (25/34) and 94% (25/27) of SDH and DDH neurons, respectively. In contrast, GABAAR-mediated mIPSCs were detected in virtually all neurons in both regions (93%, 14/15 and 100%, 18/18). Several Gly- and GABAAR properties also differed in SDH vs. DDH. GlyR-mediated mIPSC amplitude was smaller (37.1 +/- 3.9 vs. 64.7 +/- 5.0 pA; n = 25 each), decay time was slower (8.5 +/- 0.8 vs. 5.5 +/- 0.3 ms), and frequency was lower (0...Continue Reading

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Citations

May 12, 2011·British Journal of Pharmacology·Gonzalo E Yevenes, Hanns Ulrich Zeilhofer
Mar 9, 2019·Molecular Pain·J A MayhewB A Graham
Oct 24, 2019·Journal of Neural Transmission·M A GradwellB A Graham
Feb 29, 2020·Journal of Neural Transmission·Alain ArtolaRadhouane Dallel
Oct 9, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·David I Hughes, Andrew J Todd
Jul 3, 2021·Biomolecules·Hanns Ulrich ZeilhoferGonzalo E Yévenes

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Axograph
Mini Analysis Program
Primer Premier

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