PMID: 8586128Oct 1, 1995Paper

Different functional and morphological characteristics in a nonadherent subpopulation of human macrophages recovered by bronchoalveolar lavage

The European Respiratory Journal
C M SköldA Johansson

Abstract

The alveolar macrophages (AMs) constitute a morphologically and functionally heterogenous cell population. The adhesive properties of these cells are important for their role in host defence. To focus on the heterogeneity, a population of nonadherent macrophages were characterized functionally and morphologically. These cells were then compared with the total alveolar macrophage population. Alveolar cells (> 95% alveolar macrophages), were recovered by bronchoalveolar lavage (BAL) from healthy smokers. Nonadherent macrophages were separated by adhesion. The phagocytic capacity and the autofluorescent properties of the cell populations were determined in flow cytofluorometric assays. In addition, electron microscopic evaluation was performed. The alveolar macrophage adhesion to wells coated with albumin increased in a time-dependent manner. After 15 min, median 47%, interquartile range 42-52% (uncoated wells 68%, 67-72%) of the alveolar macrophages were attached; and after 60 min, 56%, 51-58% (uncoated wells 73%, 71-76%) of the alveolar macrophages were attached. The nonadherent alveolar macrophage population had less phagocytic capacity. The cell autofluorescence increased with increasing cell size and cell complexity/granulari...Continue Reading

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