Different Functions of Phylogenetically Distinct Bacterial Complex I Isozymes

Journal of Bacteriology
Melanie A SperoT J Donohue

Abstract

NADH:quinone oxidoreductase (complex I) is a bioenergetic enzyme that transfers electrons from NADH to quinone, conserving the energy of this reaction by contributing to the proton motive force. While the importance of NADH oxidation to mitochondrial aerobic respiration is well documented, the contribution of complex I to bacterial electron transport chains has been tested in only a few species. Here, we analyze the function of two phylogenetically distinct complex I isozymes in Rhodobacter sphaeroides, an alphaproteobacterium that contains well-characterized electron transport chains. We found that R. sphaeroides complex I activity is important for aerobic respiration and required for anaerobic dimethyl sulfoxide (DMSO) respiration (in the absence of light), photoautotrophic growth, and photoheterotrophic growth (in the absence of an external electron acceptor). Our data also provide insight into the functions of the phylogenetically distinct R. sphaeroidescomplex I enzymes (complex IA and complex IE) in maintaining a cellular redox state during photoheterotrophic growth. We propose that the function of each isozyme during photoheterotrophic growth is either NADH synthesis (complex IA) or NADH oxidation (complex IE). The canon...Continue Reading

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Citations

Feb 26, 2019·Frontiers in Microbiology·Angela TorresAnna Zemke
Jun 6, 2021·Bioresource Technology·Ferdinand SchmidJohannes Gescher
Jun 28, 2019·ACS Synthetic Biology·Nicholas M Tefft, Michaela A TerAvest

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