Different fusion tags affect the activity of ubiquitin overexpression on spastin protein stability.

European Journal of Histochemistry : EJH
Jianyu ZouMinghui Tan

Abstract

Spastin is one of the proteins which lead to hereditary spastic paraplegia (HSP), whose dysfunction towards microtubule severing and membrane transporting is critically important. The present study is to elucidate the mechanisms of the protein stability regulation of spastin. The ubiquitin encoding plasmids are transfected into COS-7 cells with different fusion tags including Green Fluorescent Protein (GFP), mCherry and Flag. The expression level of spastin was detected, microtubule severing activity and neurite outgrowth were quantified. The data showed that ubiquitin overexpression significantly induced the decreased expression of spastin, suppressed the activity of microtubule severing in COS-7 cells and inhibited the promoting effect on neurite outgrowth in cultured hippocampal neurons. Furthermore, when modulating the overexpression experiments of ubiquitin, it was found that relatively small tag like Flag, but not large tags such as GFP or mCherry fused with ubiquitin, retained the activity on spastin stability. The present study investigated the effects of small/large tags addition to ubiquitin and the novel mechanisms of post-transcriptional modifications of spastin on regulating neurite outgrowth, in the attempt to exp...Continue Reading

References

Aug 9, 2006·Human Molecular Genetics·Jonathan D WoodVincent T Cunliffe
Apr 9, 2009·Nature Reviews. Molecular Cell Biology·Brenda A Schulman, J Wade Harper
Jun 6, 2009·Annual Review of Biochemistry·Raymond J Deshaies, Claudio A P Joazeiro
Oct 24, 2009·Nature Reviews. Molecular Cell Biology·Yihong Ye, Michael Rape
Jun 16, 2010·Analytical and Bioanalytical Chemistry·Tae Kyung Kim, James H Eberwine
Mar 13, 2012·Annual Review of Biochemistry·Fuqiang GengWilliam P Tansey
Apr 10, 2012·Nature Methods·Dirk LandgrafJohan Paulsson
May 11, 2012·Journal of Cellular Biochemistry·Chuanling ZhangXu Ma
Jul 31, 2013·The Journal of Cell Biology·Rachel AllisonEvan Reid
Jan 31, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joanna M SolowskaPeter W Baas
Feb 4, 2014·Journal of Analytical Methods in Chemistry·Xinyu ZhaoShufang Liang
Jul 22, 2014·Protein Science : a Publication of the Protein Society·Karolina A MajorekWladek Minor
Jun 22, 2015·Brain : a Journal of Neurology·Joanna M Solowska, Peter W Baas
Mar 26, 2016·Cell Research·Kirby N Swatek, David Komander
Apr 28, 2017·Open Biology·Piotr BragoszewskiAgnieszka Chacinska
Dec 1, 2017·The Journal of Biological Chemistry·Paolo Grumati, Ivan Dikic
Feb 27, 2018·Handbook of Clinical Neurology·Craig Blackstone
Mar 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yin-Wei KuoJonathon Howard
May 1, 2019·Trends in Biotechnology·Giel VandemoorteleKris Gevaert
Jun 23, 2019·The Journal of Cell Biology·Chi-Lun ChangJennifer Lippincott-Schwartz
Jul 10, 2019·Cells·Alessandra PisciottaniCinzia Rinaldo
Oct 7, 2019·Cellular and Molecular Life Sciences : CMLS·James W ConnellEvan Reid
Nov 22, 2019·Parkinsonism & Related Disorders·Zeyu ZhuLi Cao
Oct 28, 2020·Life Science Alliance·Francesca SardinaCinzia Rinaldo
Nov 17, 2020·Frontiers in Cellular Neuroscience·Tao JiangMinghui Tan

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