Different growth hormone-receptor interactions mediate insulin-like and lipolytic responses of rat adipose tissue

Endocrinology
S R ChipkinH M Goodman

Abstract

The GH receptor in adipocytes is a glycoprotein that has a half-life of less than 1 h. After 2 h of treatment with the alkaloid swainsonine, which interferes with carbohydrate processing, virtually all of the GH receptors on the surface of adipocytes are replaced with receptors whose carbohydrate side-chains are incomplete. We examined the effects of swainsonine on the responsiveness of adipose tissue to GH to determine whether these receptors, which bind GH normally, retain biological competence. In the concentration range of 100-300 ng/ml human (h) GH rapidly evokes insulin-like responses in adipose tissue or adipocytes that have been deprived of GH for at least 3 h. hGH, at concentrations ranging from 1-10 ng/ml, also increases lipolysis after a delay of at least 2 h. Pretreatment with 50 micrograms/ml swainsonine failed to influence insulin-like responsiveness to hGH, as judged by increased glucose oxidation, but nearly completely abolished the lipolytic response. Pretreatment with swainsonine, however, did not reduce lipolysis in response to isoproterenol, suggesting that signal transmission rather than the lipolytic apparatus per se had been affected. To determine whether the same receptors mediate lipolytic and insulin-l...Continue Reading

Citations

Jul 1, 1992·Molecular and Cellular Endocrinology·R J FiddesT E Adams
Sep 1, 1993·Molecular and Cellular Endocrinology·B CarlssonH Billig
Dec 1, 1994·Acta Paediatrica. Supplement·M WabitschW Teller
Apr 6, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Helene Nørrelund
Aug 6, 2002·The Journal of Biological Chemistry·Shaonin JiJoseph L Messina

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