Different human tenascin-C variants in the extracellular matrix of cultured human fibroblasts

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
A SiriL Zardi

Abstract

Using an immunoadsorbent prepared with a monoclonal antibody specific for the high molecular mass isoform of human tenascin-C, we purified tenascin-C molecules containing at least one large subunit from the extracellular matrix of cultured normal human fibroblasts. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting analyses have shown that both high and low molecular mass subunits are present in these tenascin-C preparations. Because the monoclonal antibody used is able to bind only the high molecular mass isoform, the present data show that part of the tenascin-C present in the fibroblast extracellular matrix is made up of heterohexameric molecules.

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Citations

Mar 14, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·A Joester, A Faissner
Sep 29, 2004·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Falk WehrhanStefan Schultze-Mosgau
Aug 23, 2003·Blood·Laura BorsiLuciano Zardi

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