Different in vitro and in vivo targeting properties of the transit peptide of a chloroplast envelope inner membrane protein.

The Journal of Biological Chemistry
M D Silva-FilhoM Boutry

Abstract

The triose phosphate 3-phosphoglycerate phosphate translocator (TPT) is a chloroplast envelope inner membrane protein whose transit peptide has structural properties typical of a mitochondrial presequence. To study the TPT transit peptide in more detail, we constructed two chimeric genes encompassing the TPT transit peptide and either 5 or 23 amino-terminal residues of the mature TPT, both linked to the reporter chloramphenicol acetyltransferase (cat) gene. The precursors were synthesized in vitro and translocated to and processed in purified plant mitochondria. However, this import was not specific since both precursors were also imported into isolated chloroplasts. To extend this analysis in vivo, the chimeric genes were introduced into tobacco by genetic transformation. Analysis of CAT distribution in subcellular fractions of transgenic plants did not confirm the data obtained in vitro. With the construct retaining only 5 residues of the mature TPT, CAT was found in the cytosolic fraction. Extension of the TPT transit peptide to 23 residues of the mature TPT allowed specific import and processing of CAT into chloroplasts. These results indicate that, despite its unusual structure, the TPT transit peptide is able to target a ...Continue Reading

References

Nov 13, 1991·Biochimica Et Biophysica Acta·A Douwe de Boer, P J Weisbeek
Jan 14, 1991·FEBS Letters·G von Heijne, K Nishikawa
Apr 1, 1989·European Journal of Biochemistry·G von HeijneR G Herrmann
Nov 26, 1984·Nucleic Acids Research·M Bevan
Sep 1, 1995·The Journal of Biological Chemistry·S BrinkU I Flügge
Aug 23, 1993·FEBS Letters·T LithgowN J Hoogenraad

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Citations

Jul 20, 2004·Planta·Cathrin ClausenJürgen Soll
Feb 14, 2009·Molecular Genetics and Genomics : MGG·Carolina V MorganteMarcio C Silva-Filho
Aug 16, 2002·Biochimica Et Biophysica Acta·Paul Jarvis, Jürgen Soll
May 18, 2000·Plant Science : an International Journal of Experimental Plant Biology·R M BarataM C Silva-Filho
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·B HedtkeA Weihe
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Charlotta RudheElzbieta Glaser
Nov 15, 2000·Biological Chemistry·U C Vothknecht, J Soll
Sep 14, 2001·Biochemical and Biophysical Research Communications·M Fujiwara, S Yoshida
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·S GálvezM Hodges
Oct 18, 2014·Journal of Experimental Botany·Monika W MurchaJames Whelan
Feb 15, 2008·Plant Physiology and Biochemistry : PPB·Andrew J PerryPaul R Gooley
Oct 23, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Andrzej BodyłJohn W Stiller
Sep 8, 2009·Journal of Molecular Biology·Anna-Karin BerglundElzbieta Glaser
Jan 9, 1999·The Journal of Biological Chemistry·M TanudjiJ Whelan
Dec 26, 2001·Biochimica Et Biophysica Acta·P Jarvis, J Soll
Dec 26, 2001·Biochimica Et Biophysica Acta·N Peeters, I Small

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