PMID: 3743888Sep 1, 1986Paper

Different interaction of estradiol and antiestrogens with the estrogen receptor of rat uterus

Molecular and Cellular Endocrinology
J C FayeF Bayard

Abstract

Cytosoluble estradiol-receptor (ER) complexes obtained from uteri of castrated rats show a 4.3 S sedimentation coefficient when analysed by sucrose gradient in high salt buffer both in the absence and presence of 0.1 M ammonium thiocyanate. After incubation with nuclei, the complexes sediment at 4.3 S and at 3.2 S, in the absence and presence of 0.1 M ammonium thiocyanate respectively. This structural modification of ER is also confirmed by gel filtration analysis. In similar conditions hydroxytamoxifen-ER complexes apparently do not undergo such a modification. However, increasing the molarity of the chaotropic ion up to 0.5 M shows that, in fact, the modification occurs. Sodium dodecylsulfate-polyacrylamide gel electrophoresis analysis of covalently linked tamoxifen aziridine-ER 'complexes' confirms that the nuclear treatment reduces the apparent molecular weight of ER from 62 000 to 47 000. These data demonstrate that, in vitro, nuclear ER is cleaved into a smaller molecular form and that the receptor fragments can be held together by the triphenylethylene antiestrogen hydroxytamoxifen.

References

Nov 14, 1979·Biochemical and Biophysical Research Communications·R L Sutherland, M S Foo
Feb 16, 1973·Nature·G C ChamnessW L McGuire
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J C FayeF Bayard
Feb 23, 1984·Nature·W V WelshonsJ Gorski
Jan 1, 1982·Molecular and Cellular Endocrinology·R L Sutherland, L C Murphy
Apr 29, 1980·Biochemical and Biophysical Research Communications·J C FayeF Bayard
Oct 1, 1980·Molecular and Cellular Endocrinology·J L Borgna, H Rochefort

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