Different interactions of cardiac and skeletal muscle ryanodine receptors with FK-506 binding protein isoforms

The American Journal of Physiology
S BargS Fleischer

Abstract

In the present study, we compare functional consequences of dissociation and reconstitution of binding proteins FKBP12 and FKBP12.6 with ryanodine receptors from cardiac (RyR2) and skeletal muscle (RyR1). The skeletal muscle RyR1 channel became activated on removal of endogenously bound FKBP12, consistent with previous reports. Both FKBP12 and FKBP12.6 rebind to FKBP-depleted RyR1 and restore its quiescent channel behavior by altering ligand sensitivity, as studied by single-channel recordings in planar lipid bilayers, and macroscopic behavior of the channels (ryanodine binding and net energized Ca2- uptake). By contrast, removal of FKBP12.6 from the cardiac RyR2 did not modulate the function of the channel using the same types of assays as for RyR1. FKBP12 or FKBP12.6 had no effect on channel activity of FKBP12.6-depleted cardiac RyR2, although FKBP12.6 rebinds. Our studies reveal important differences between the two ryanodine receptor isoforms with respect to their functional interaction with FKBP12 and FKBP12.6.

References

Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S FleischerE A Fleer
Jan 1, 1989·Annual Review of Biophysics and Biophysical Chemistry·S Fleischer, M Inui
Nov 30, 1993·Annals of the New York Academy of Sciences·G WiederrechtN Sigal
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·S R ChenD H MacLennan
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·Y Ogawa
Jan 28, 1994·Biochemical and Biophysical Research Communications·A P TimermanS Fleischer
Aug 23, 1996·The Journal of Biological Chemistry·A P TimermanS Fleischer

❮ Previous
Next ❯

Citations

Jun 20, 1998·The Journal of Biological Chemistry·L H JeyakumarS Fleischer
Apr 1, 2000·Clinical and Experimental Pharmacology & Physiology·G D Lamb
Aug 14, 2009·Journal of Veterinary Internal Medicine·M A OyamaC A Reynolds
Jul 13, 2012·American Journal of Physiology. Cell Physiology·Maura PortaJulio A Copello
Nov 11, 2017·The Journal of General Physiology·Gerhard Meissner
Dec 4, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ximin ChiNieng Yan
Oct 13, 2000·American Journal of Physiology. Cell Physiology·T ObaM Yamaguchi
Dec 22, 1999·Annals of the New York Academy of Sciences·D M BersE McCall
Dec 19, 2001·The Anatomical Record·Laurel M PattersonHans-Rudolf Berthoud
Oct 21, 2018·The Journal of Biological Chemistry·Le XuGerhard Meissner
Jun 17, 1998·The Journal of Physiology·A Zahradníková, L G Meszáros
Mar 23, 2004·The Journal of Biological Chemistry·Eun Hui LeeDo Han Kim
Mar 22, 2002·Nature·Hong-Bo XinSidney Fleischer
Apr 21, 2010·The Journal of Biological Chemistry·Razvan L CorneaBradley R Fruen
Sep 25, 2002·Physiological Reviews·Michael Fill, Julio A Copello
Apr 13, 2007·Physiological Reviews·Henk E D J Ter Keurs, Penelope A Boyden
Dec 24, 2010·Cardiovascular Therapeutics·Elisa VenturiRebecca Sitsapesan
Dec 7, 2014·Science China. Life Sciences·Yan-Ting ZhaoHéctor H Valdivia
Apr 25, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·Aimo KanntHeinz Gögelein
Mar 10, 2000·American Journal of Physiology. Heart and Circulatory Physiology·W H DuBellT B Rogers
Feb 24, 2001·Journal of Neurochemistry·H HigashidaT Kato
Feb 14, 2012·Journal of Cell Science·Spyros ZissimopoulosF Anthony Lai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.