Different involvement for aldolase isoenzymes in kidney glucose metabolism: aldolase B but not aldolase A colocalizes and forms a complex with FBPase

Journal of Cellular Physiology
Alejandro J YañezJuan C Slebe

Abstract

The expression of aldolase A and B isoenzyme transcripts was confirmed by RT-PCR in rat kidney and their cell distribution was compared with characteristic enzymes of the gluconeogenic and glycolytic metabolic pathway: fructose-1,6-bisphosphatase (FBPase), phosphoenol pyruvate carboxykinase (PEPCK), and pyruvate kinase (PK). We detected aldolase A isoenzyme in the thin limb and collecting ducts of the medulla and in the distal tubules and glomerula of the cortex. The same pattern of distribution was found for PK, but not for aldolase B, PEPCK, and FBPase. In addition, co-localization studies confirmed that aldolase B, FBPase, and PEPCK are expressed in the same proximal cells. This segregated cell distribution of aldolase A and B with key glycolytic and gluconeogenic enzymes, respectively, suggests that these aldolase isoenzymes participate in different metabolic pathways. In order to test if FBPase interacts with aldolase B, FBPase was immobilized on agarose and subjected to binding experiments. The results show that only aldolase B is specifically bound to FBPase and that this interaction was specifically disrupted by 60 microM Fru-1,6-P2. These data indicate the presence of a modulated enzyme-enzyme interaction between FBPas...Continue Reading

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Citations

Oct 25, 2011·Journal of Cellular Biochemistry·Romina BertinatAlejandro J Yáñez
Oct 13, 2012·Journal of Cellular Biochemistry·Rodrigo GaticaAlejandro J Yáñez
Nov 4, 2010·The Biochemical Journal·Agnieszka A KendrickKaren R Jonscher
Dec 15, 2015·Journal of Cellular Physiology·R BertinatA J Yáñez
Nov 6, 2014·Histochemistry and Cell Biology·Rodrigo GaticaAlejandro J Yáñez
Jul 14, 2018·PloS One·Varun ChhabraMaramreddy Prasanna Kumar Reddy
Jun 21, 2005·Journal of Cellular Physiology·Alejandro J YáñezJuan C Slebe
Sep 13, 2007·The Journal of Biological Chemistry·Boubacar BenzianeKamel Laghmani
Sep 12, 2018·The Journal of Clinical Endocrinology and Metabolism·Felicia GerstRobert Wagner
Nov 5, 2019·Rapid Communications in Mass Spectrometry : RCM·Han-Ju ChienChien-Chen Lai
Feb 2, 2019·World Journal of Nephrology·Deepika JainNarender Goel
Feb 2, 2019·World Journal of Nephrology·Sarojini SinghSwasti Tiwari
Jun 1, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yokananth SekarA Dean Befus
Mar 26, 2015·Development·Yuwen LiZubaida Saifudeen
May 18, 2021·World Journal of Diabetes·Rajni Sharma, Swasti Tiwari

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