Abstract
Glioblastoma (GB) has a poor prognosis, despite current multimodality treatment. Beside surgical resection, adjuvant ionizing radiation (IR) combined with Temozolomide (TMZ) drug administration is the standard therapy for GB. This currently combined radio-chemotherapy treatment resulted in glial tumor cell death induction, whose main molecular death pathways are still not completely deciphered. In this study, the autophagy process was investigated, and in vitro modulated, in two different GB cell lines, T98G and U373MG (known to differ in their radiosensitivity), after IR or combined IR/TMZ treatments. T98G cells showed a high radiosensitivity (especially at low and intermediate doses), associated with autophagy activation, assessed by Beclin-1 and Atg-5 expression increase, LC3-I to LC3-II conversion and LC3B-GFP accumulation in autophagosomes of irradiated cells; differently, U373MG cells resulted less radiosensitive. Autophagy inhibition, using siRNA against BECN1 or ATG-7 genes, totally prevented decrease in viability after both IR and IR/TMZ treatments in the radiosensitive T98G cells, confirming the autophagy involvement in the cytotoxicity of these cells after the current GB treatment, contrary to U373MG cells. However, ...Continue Reading
References
Aug 1, 1996·Carcinogenesis·W BurschR S Hermann
Aug 6, 2000·Cell and Tissue Research·M Verheij, H Bartelink
Feb 15, 2001·International Journal of Radiation Oncology, Biology, Physics·M C JoinerI Turesson
Feb 21, 2003·Journal of Neurosurgery·Kevin C YaoIsabelle M Germano
Nov 26, 2003·The Journal of Clinical Investigation·Xueping QuBeth Levine
Jan 10, 2004·Cell Death and Differentiation·T KanzawaS Kondo
Feb 19, 2004·The Journal of Biological Chemistry·Francesca ScarlattiPatrice Codogno
Apr 8, 2004·Developmental Cell·Beth Levine, Daniel J Klionsky
Jun 29, 2004·Journal of Neuro-oncology·Tadashi KomataRyuichi Tanaka
Aug 4, 2004·Cancer Research·Nicole FehrenbacherMarja Jäättelä
Dec 14, 2004·Oncogene·Takao KanzawaSeiji Kondo
Apr 19, 2005·Cancer Research·Hayato TakeuchiSeiji Kondo
Oct 4, 2005·The Journal of Clinical Investigation·Beth Levine, Junying Yuan
May 20, 2006·Neurosurgical Focus·Florence Lefranc, Robert Kiss
Jul 29, 2006·Autophagy·Isei TanidaEiki Kominami
Sep 27, 2006·Oncogene·A IwamaruS Kondo
Feb 24, 2007·Radiation Research·S A KruegerB Marples
Aug 25, 2007·Nature Reviews. Molecular Cell Biology·M Chiara MaiuriGuido Kroemer
Sep 6, 2007·International Journal of Oncology·Keishi FujiwaraYasuko Kondo
Jan 12, 2008·Autophagy·Daniel J KlionskyRussell L Deter
Dec 3, 2008·Journal of Neurosurgery·Yoshifumi TsuboiShunro Endo
May 12, 2009·Acta Biochimica Et Biophysica Sinica·Wenzhuo ZhuangZhongqin Liang
Jun 6, 2009·Journal of Cellular Biochemistry·Jung-Mu HurDongho Kim
Sep 1, 2009·Clinical Neurology and Neurosurgery·Deok-Joo RheeDo-Hyun Nam
Nov 26, 2009·International Journal of Radiation Oncology, Biology, Physics·Anthony J ChalmersSusan C Short
Jan 15, 2011·Biogerontology·A A MoskalevM V Shaposhnikov
Jan 29, 2011·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Manabu NatsumedaYukihiko Fujii
Mar 9, 2011·International Journal of Cancer. Journal International Du Cancer·Wenzhuo ZhuangZhongqin Liang
Apr 12, 2011·Autophagy·Giulia BarbieriSergio Comincini
Citations
Feb 6, 2013·PloS One·Anna V KnizhnikBernd Kaina
Feb 4, 2014·Cellular Immunology·Cristina E GallegosEdgardo D Carosella
Sep 12, 2014·Neuro-oncology·Yuanyuan ChenDaru Lu
Jun 7, 2014·International Journal of Molecular Sciences·Khushboo SharmaDavid A Gewirtz
Mar 29, 2014·Journal of Cellular Physiology·Nicoletta MarchesiSergio Comincini
Apr 3, 2014·Journal of Cellular Physiology·Silvia PalumboLuigi Pirtoli
Feb 3, 2016·Journal of Experimental & Clinical Cancer Research : CR·Yuanliang YanZhicheng Gong
Nov 11, 2015·Autophagy·Yuntao LuSongtao Qi
May 16, 2012·Journal of Cellular Physiology·Silvia Palumbo, Sergio Comincini
Jul 19, 2014·Neurological Research·Danop NanegrungsunkSiriporn C Chattipakorn
Sep 4, 2014·Radiation Research·David A Gewirtz
Jun 25, 2013·Cancer Biology & Therapy·Sergio CominciniClelia Miracco
Mar 18, 2015·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Marco B E SchaafKasper M A Rouschop
Mar 31, 2015·BioMed Research International·Paolo TiniLuigi Pirtoli
Sep 16, 2014·Experimental Cell Research·Xiaopeng YuanFenju Liu
Feb 7, 2015·Cancer Letters·S FerrandonC Rodriguez-Lafrasse
Dec 20, 2014·Pathology, Research and Practice·Haiwei WuDongsheng Zhang
Apr 3, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ilkka LiikanenAkseli Hemminki
Jan 21, 2017·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Vincent Hung-Shu ChangHui-Ju Ch'ang
Dec 12, 2013·The Journal of International Medical Research·Wei ShenJie-Sheng Zheng
Nov 30, 2013·Pharmaceuticals·Yulian P RamirezAlonzo H Ross
Jun 15, 2018·Cancer Science·Ha Thi VuAtsushi Hirao
Mar 8, 2018·Current Neuropharmacology·María Jesús Ramírez-Expósito, José Manuel Martínez-Martos
Aug 9, 2017·Journal of Cancer Research and Clinical Oncology·Yong XinGuan Jiang
Oct 12, 2018·Cell Death & Disease·Xiaoxiong WangShiguang Zhao
Sep 22, 2019·Cells·Wanyeon KimBuHyun Youn
Feb 7, 2020·American Journal of Physiology. Cell Physiology·Luigi PirtoliClelia Miracco
Nov 18, 2018·Oncotarget·Juliette ServanteRichard Grundy
Mar 23, 2017·Radiation Oncology·Shing Yau TamHelen Ka Wai Law
Mar 9, 2019·Cancers·Barbara ColellaSabrina Di Bartolomeo
Jun 20, 2020·Cellular and Molecular Neurobiology·Zatollah AsemiOmid Reza Tamtaji
Jul 5, 2018·Journal of Cancer Research and Clinical Oncology·Hongbin LiQiang Li
Mar 23, 2019·Scientific Reports·Vimal PandeyPhanithi Prakash Babu
Aug 11, 2019·Current Molecular Pharmacology·Lei LiXiu-Sheng He
Jul 12, 2020·Biomolecules·Mina AlimohammadiAlireza Rafiei
Aug 3, 2017·Molecular Medicine Reports·Yan LinZhiguang Tu
Feb 26, 2021·Autophagy·Tengfei HuangChao Yan
Feb 24, 2021·Aging·Jia ShiJun Dong
Aug 28, 2021·International Journal of Molecular Sciences·Valerio NardoneLuigi Pirtoli