Different levels of testicular organization during gonadal differentiation in B6.Y(Tir) mice manifesting sex reversal

Cell Biology International
Nivia Rocio Antonio-Rubio, Norma Moreno-Mendoza

Abstract

B6.Y(Tir) (mice with Y chromosome from a strain in Tirano, Italy, and autosomes and X-chromosomes from the B6 strain) mice provide an excellent model for analysing sex development that occurs during gonadal differentiation; however, the molecular mechanisms that contribute to sex reversal are unclear. Our aim has been to establish which molecular events participate in this sex reversal. The pattern of gene expression related to testicular [Sry (sex-determining region of the Y chromosome), Sox9 (Sry-related high-mobility group box gene 9) and Mis (Müllerian-inhibiting substance)] and ovarian [Wnt4 (Wingless-type MMTV (murine-mammary-tumour virus) integration site family, member 4), Rspo1 (cysteine-rich secretory protein containing a thrombospondin type 1 repeat) and Stra8 (stimulated by retinoic acid gene 8)] differentiation was analysed by applying immunofluorescence and real-time RT-PCR (reverse transcription-PCR), focusing on XY gonads from the B6.Y(Tir) mouse, but also analysing the normal strains CD-1 and C57BL/6J (B6). The expression of genes related to the process of sexual differentiation was altered in the case of the B6.Y(Tir) strain, both at the transcript and protein level, inducing differentiation of ovaries and ovo...Continue Reading

References

Aug 1, 1992·The Journal of Urology·A M Houle, T Taketo
May 9, 1991·Nature·P KoopmanR Lovell-Badge
Jan 1, 1994·Developmental Genetics·H Merchant-LariosT Taketo
Jul 1, 1997·Developmental Biology·A McLaren, D Southee
May 16, 2001·Genesis : the Journal of Genetics and Development·C H Lee, T Taketo
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 19, 2003·Biology of Reproduction·Norma Moreno-MendozaHoracio Merchant-Larios
Feb 1, 2005·Developmental Biology·Monica Bullejos, Peter Koopman
Dec 17, 2005·Cell Cycle·Kyung-Ah KimWalter D Funk
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jana KoubovaDavid C Page
Oct 13, 2006·Gene Expression Patterns : GEP·Ju-Suk NamJeong Kyo Yoon
Oct 17, 2006·Nature Genetics·Pietro ParmaGiovanna Camerino
May 15, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Akihiro FujinoNelson A Arango
Aug 2, 2007·Molecular Endocrinology·Leo DiNapoli, Blanche Capel
Dec 19, 2007·Human Mutation·Sara TomaselliPaola Grammatico
Feb 6, 2008·Human Molecular Genetics·Kazuma TomizukaMakoto Kakitani
Feb 6, 2008·Human Molecular Genetics·Anne-Amandine ChassotMarie-Christine Chaboissier
Jul 12, 2008·Human Molecular Genetics·Danielle M MaatoukBlanche Capel
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ericka L AndersonDavid C Page
Nov 28, 2008·Development·Ryuji HiramatsuYoshiakira Kanai
Mar 10, 2009·Mechanisms of Development·Dagmar WilhelmPeter Koopman
Apr 21, 2009·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yun-Fai Chris Lau, Yunmin Li
May 20, 2011·Differentiation; Research in Biological Diversity·Stephanie ParkTeruko Taketo

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.