Different macrophages equally induce EMT in endometria of adenomyosis and normal

Reproduction : the Official Journal of the Society for the Study of Fertility
Min AnGuoyun Wang

Abstract

Endometrial cells and microenvironment are two important factors in the pathogenesis of adenomyosis. Our previous study demonstrated that macrophages can induce eutopic epithelial cells of adenomyosis to suffer from epithelial-mesenchymal transition (EMT). The aim of this study is to detect whether macrophages interacting with epithelial cells equally induce the EMT process in normal and eutopic endometria of healthy and adenomyotic patients; and whether macrophages parallelly polarize to M2. We investigated the expression levels of epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), cytokeratin7 (CK7), vimentin, transforming growth factor-β1 (TGFB1), SMAD3 and pSMAD3 using immunohistochemistry and western blot, and then estimated the genetic levels of CD163, IL10 and MMP12 using real-time quantitative polymerase chain reaction (RT-PCR) in macrophages. Eutopic and normal endometrial tissues were obtained from 20 patients with adenomyosis and 11 control patients without adenomyosis, respectively. The immunohistochemical analysis shows distinct EMT in eutopic endometria in secretory phase; the expression levels of TGFB1, SMAD3 and pSMAD3 that indicate signal pathway of EMT were also higher in secretory phase. Macropha...Continue Reading

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Citations

Sep 11, 2019·Human Reproduction Update·Marissa J HarmsenJudith A F Huirne
Feb 7, 2020·International Journal of Molecular Sciences·Xuezi TianMarie-Louise van der Hoorn
Oct 23, 2020·Reproductive Sciences·Christina Anna StratopoulouMarie-Madeleine Dolmans
Oct 26, 2020·Human Reproduction Update·M BourdonC Chapron
May 7, 2021·Journal of Gynecology Obstetrics and Human Reproduction·Mohummad Hasan Raza RajaRehana Rehman
Oct 18, 2021·Gynécologie, Obstétrique, Fertilité & Sénologie·M BourdonC Chapron

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