Different mechanisms mediate the rejection of porcine neurons and endothelial cells transplanted into the rat brain

Xenotransplantation
Séverine RémyPhilippe Brachet

Abstract

In order to investigate the early cellular responses mediating xenograft rejection in the brain, porcine aortic endothelial cells (PAEC) or porcine fetal mesencephalic neurons (PNEU) were transplanted into the striatum of LEW.1A rats. PAEC were detected with a specific anti-beta1 integrin antibody, and PNEU with an anti-porcine neurofilament antibody, or an antibody recognizing the NeuN antigen. PAEC grafts were massively infiltrated within 24 h by OX42-positive cells, which may correspond to polymorphonuclear (PMN) cells or macrophages. At that moment, the graft contained numerous cells expressing the inducible isoform of NO-synthase (iNOS). Infiltration by ED1-positive macrophages was effective after three days. The beta1-integrin labeling decreased from that time-point to day 7 post-implantation, and vanished after 11 days. Although some OX8-positive cells were present around the graft as soon as 3 days after transplantation, cells expressing the T-cell receptor (TCR)-beta chain infiltrated the graft after 7 days and their number remained low. A strong, diffuse OX8-and ED1-positive immunoreactive material remained in the scar up to the third week. In striking contrast, PNEU grafts remained poorly infiltrated by OX42- or ED1-...Continue Reading

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Citations

Oct 6, 2005·Transgenic Research·Caroline MartinBernard Vanhove
Nov 26, 2010·Immunology and Cell Biology·Irene BergwerfPeter Ponsaerts
Feb 10, 2010·Journal of Cellular and Molecular Medicine·Julien RossignolLaurent Lescaudron
May 6, 2011·Immunotherapy·Delphine Michel-MonigadonPhilippe Naveilhan
Aug 29, 2014·Stem Cells International·Gregory E TullisMark D Kirk
Mar 19, 2011·Current Opinion in Organ Transplantation·Xavier LévêqueIsabelle Neveu
Jul 26, 2015·International Journal of Surgery·Mohamed B Ezzelarab, David K C Cooper
Mar 13, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·E MathieuxI Neveu
Mar 10, 2005·Experimental Neurology·Benoît MelchiorPhilippe Brachet
Sep 12, 2014·Xenotransplantation·Mohamed B EzzelarabDavid K Cooper
May 18, 2010·Experimental Neurology·Delphine Michel-MonigadonPhilippe Naveilhan
Aug 12, 2005·Neurorehabilitation and Neural Repair·W-L Kuan, R A Barker
Oct 29, 2003·Cell Transplantation·Suchitra Sumitran-HolgerssonJan Holgersson
Sep 15, 2006·Cell Transplantation·Jan KoopmansMichiel Staal
Dec 17, 2005·Cell Transplantation·Caroline MartinPhilippe Brachet
Jun 9, 2009·Cell Transplantation·Jean-François BrunetJocelyne Bloch
Mar 31, 2007·Cell Death and Differentiation·E Syková, P Jendelová

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