PMID: 8600982Mar 15, 1996Paper

Different mechanisms of formation of glutathione-protein mixed disulfides of diamide and tert-butyl hydroperoxide in rat blood

Biochimica Et Biophysica Acta
P Di SimplicioR Rossi

Abstract

The mechanisms of glutathione-protein mixed disulfide (GSSP) formation caused by diamide and tert-butyl hydroperoxide were studied in rat blood after in vitro treatment in the 0.3-4 mM dose range. tert-Butyl hydroperoxide formed GSSP, via GSSG, according to the reaction, GSSG + PSH --> GSSP + GSH, whereas diamide reacted first with protein SH groups, giving PS-diamide adducts and then, after reaction with GSH, GSSP. Moreover, after diamide treatment, GSSP patterns were characterized by a much slower or irreversible dose-related return to basal levels in comparison with those observed with tert-butyl hydroperoxide, always reversible. Experiments with purified hemoglobin revealed the existence of a large fraction of protein SH groups which formed GSSP and had a higher reactivity than GSH. Experiments on glucose consumption and role of various erythrocyte enzymes, carried out to explain the inertness of GSSP to reduction after treatment of blood with diamide, were substantially negative. Other tests carried out to confirm the efficiency of the enzymatic machinery of blood samples successively treated with diamide and tert-butyl hydroperoxide, indicated that GSSP performed by diamide was difficult to reduce, whereas those generated...Continue Reading

References

Aug 1, 1977·European Journal of Biochemistry·N S KosowerR L Koppel
Aug 23, 1976·Biochemical and Biophysical Research Communications·R A Lawrence, R F Burk
Dec 1, 1986·Toxicology and Industrial Health·R BrigeliusJ R Bend
Jan 1, 1984·Methods in Enzymology·H F Gilbert
Jan 1, 1984·Methods in Enzymology·H Aebi
Feb 11, 1982·Biochemical and Biophysical Research Communications·J K Stoops, S J Wakil
May 15, 1981·European Journal of Biochemistry·S G CondòM Brunori
Jun 15, 1990·Physical Review. B, Condensed Matter·W C Mitchel, R E Perrin
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Jan 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R VignaniP Di Simplicio

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Citations

Mar 15, 2001·Chemico-biological Interactions·F GianneriniP Di Simplicio
Jul 7, 2005·Antioxidants & Redox Signaling·Paolo Di SimplicioAnna Di Stefano
Jan 5, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Suaib LuqmanDebabrata Chanda
Nov 1, 2015·Biochemical and Biophysical Research Communications·Sergey M KorotkovIrina V Brailovskaya
Jul 24, 1998·Archives of Biochemistry and Biophysics·P Di SimplicioR Rossi
Feb 27, 2013·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Hao PanHuidi Jiang
Aug 11, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kristen HentyThomas Brittain

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