Different media and supplements modulate the clonogenic and expansion properties of rabbit bone marrow mesenchymal stem cells.

BMC Research Notes
Simone LapiFabrizio Scatena

Abstract

Rabbits provide an excellent model for many animal and human diseases, such as cardiovascular diseases, for the development of new vaccines in wound healing management and in the field of tissue engineering of tendon, cartilage, bone and skin.The study presented herein aims to investigate the biological properties of bone marrow rabbit MSCs cultured in different conditions, in order to provide a basis for their clinical applications in veterinary medicine. MSCs were isolated from 5 New Zealand rabbits. Fold increase, CFU number, doubling time, differentiation ability and immunophenotype were analyzed.With the plating density of 10 cells/cm2 the fold increase was significantly lower with DMEM-20%FCS and MSCs growth was significantly higher with alphaMEM-hEGF. The highest clonogenic ability was found at 100 cell/cm2 with MSCBM and at 10 cell/cm2 with M199. Both at 10 and 100 cells/cm2, in alphaMEM medium, the highest CFU increase was obtained by adding bFGF. Supplementing culture media with 10%FCS-10%HS determined a significant increase of CFU. Our data suggest that different progenitor cells with differential sensitivity to media, sera and growth factors exist and the choice of culture conditions has to be carefully considered f...Continue Reading

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Citations

Jul 28, 2012·Cell and Tissue Research·P SreejitR S Verma
Dec 12, 2013·BMC Research Notes·Tsung-Han LeeTao-Chen Lee
Oct 24, 2013·In Vitro Cellular & Developmental Biology. Animal·A BakhtinaT Livingston Arinzeh
Jul 6, 2013·Apoptosis : an International Journal on Programmed Cell Death·Maria Serena PiccinnoMassimo Dominici
Apr 4, 2015·Journal of Animal Science and Biotechnology·Nuradilla Mohamad-FauziJames D Murray
Nov 8, 2014·PloS One·Tao-Chen LeeMartin Hsiu-Chu Lin
Jul 12, 2019·Journal of Applied Biomaterials & Functional Materials·Dong Hwan KimChung-Hwan Baek
Dec 14, 2011·The International Journal of Artificial Organs·Elisa FiorentiniGabriela Ciapetti
Aug 3, 2021·Annals of Medicine and Surgery·Khan Sharun Amarpal
Sep 4, 2020·Chemistry and Physics of Lipids·Camila Gonzaga da SilvaFernando Gonçalves de Almeida

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Methods Mentioned

BETA
FCS
light microscopy
FACS

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