Different metabolic features of Bacteroides fragilis growing in the presence of glucose and exopolysaccharides of bifidobacteria

Frontiers in Microbiology
David Rios-CovianClara G de los Reyes-Gavilán

Abstract

Bacteroides is among the most abundant microorganism inhabiting the human intestine. They are saccharolytic bacteria able to use dietary or host-derived glycans as energy sources. Some Bacteroides fragilis strains contribute to the maturation of the immune system but it is also an opportunistic pathogen. The intestine is the habitat of most Bifidobacterium species, some of whose strains are considered probiotics. Bifidobacteria can synthesize exopolysaccharides (EPSs), which are complex carbohydrates that may be available in the intestinal environment. We studied the metabolism of B. fragilis when an EPS preparation from bifidobacteria was added to the growth medium compared to its behavior with added glucose. 2D-DIGE coupled with the identification by MALDI-TOF/TOF evidenced proteins that were differentially produced when EPS was added. The results were supported by RT-qPCR gene expression analysis. The intracellular and extracellular pattern of certain amino acids, the redox balance and the α-glucosidase activity were differently affected in EPS with respect to glucose. These results allowed us to hypothesize that three general main events, namely the activation of amino acids catabolism, enhancement of the transketolase reac...Continue Reading

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Citations

Jul 16, 2016·BMC Microbiology·David Rios-CovianClara G de Los Reyes-Gavilán
Apr 11, 2018·Nature Reviews. Clinical Oncology·Bertrand RoutyGuido Kroemer
Mar 23, 2017·Frontiers in Microbiology·David Rios-CovianClara G de Los Reyes-Gavilan
Nov 14, 2017·Experimental Dermatology·LiRong TanCong Peng
Jul 2, 2017·Digestive Diseases and Sciences·Surbhi AggarwalJaishree Paul
Aug 14, 2020·The Journal of Clinical Endocrinology and Metabolism·Xinhuan SuQunye Zhang
Feb 2, 2021·Bioscience of Microbiota, Food and Health·Shin Kurihara
Dec 31, 2020·Microorganisms·Sabrina DurantiFrancesca Turroni
Jun 24, 2017·Microbiology Spectrum·Claudio Hidalgo-CantabranaAbelardo Margolles
Nov 9, 2021·Clinical & Experimental Metastasis·Charlotte HenstraAnika Nagelkerke

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Methods Mentioned

BETA
Protein Assay
electrophoresis
PCR
fluorescence spectroscopy
Fluorescence

Software Mentioned

SPSS
- PC
2DImageMaster
Empower

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