Different methods of identifying new antigenic epitopes of human papillomavirus type 16 E6 and E7 proteins

Clinical and Diagnostic Laboratory Immunology
Mayumi NakagawaAnna-Barbara Moscicki

Abstract

Human papillomavirus (HPV) infection is the most common cause of sexually transmitted viral infection and is the main cause of cervical cancer. Identification of HPV T-cell epitopes would be instrumental not only in our understanding of the protective immune response but also in the development of vaccines and immunotherapies. In contrast to viruses which cause systemic infection, identification of HPV epitopes is technically challenging because HPV causes a localized mucosal infection and the frequency of pathogen-specific T lymphocytes in peripheral blood is expected to be low. Here we describe three new antigenic epitopes (E7 7-15 [TLHEYMLDL], E6 52-61 [FAFRDLCIVY], and E7 79-87 [LEDLLMGTL]) of HPV 16 E6 and E7 proteins which have oncogenic activities. E7 7-15 was identified among peptides previously shown to bind to human leukocyte antigen (HLA)-A2.1 molecule, but it was found likely to be restricted by the HLA-B48 molecule. E6 52-61 (likely to be restricted by HLA-B57) and E7 79-87 (likely to be restricted by HLA-B60) were detected, based on the magnitude of the T-cell immune responses, in another individual. In particular, T-cell clones specific for the E6 52-61 epitope were isolated effectively by magnetically selecting ...Continue Reading

Citations

Feb 27, 2008·Archives of Dermatological Research·Yan XuHao Cheng
Nov 17, 2006·Journal of Virology·Mayumi NakagawaAnna-Barbara Moscicki
Mar 22, 2012·Journal of Visualized Experiments : JoVE·Xuelian WangMayumi Nakagawa
Oct 24, 2007·International Journal of Cancer. Journal International Du Cancer·Sytse J PiersmaSjoerd H van der Burg
Mar 28, 2008·International Journal of Cancer. Journal International Du Cancer·Karen J ThomasStephen Man
Sep 22, 2015·PloS One·Liliane Maria Fernandes de OliveiraPaulo Lee Ho
Sep 3, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Nirmala Subramanian, Sudandiradoss Chinnappan
Aug 25, 2020·International Journal of Peptide Research and Therapeutics·Bahareh BahmaniAmir-Hassan Zarnani

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