Different microRNA profiles reveal the diverse outcomes induced by EV71 and CA16 infection in human umbilical vein endothelial cells using high-throughput sequencing

PloS One
Jie SongLongding Liu

Abstract

Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) remain the predominant pathogens in hand, foot, and mouth disease (HFMD), but the factors underlying the pathogenesis of EV71 and CA16 infections have not been elucidated. Recently, the functions of microRNAs (miRNAs) in pathogen-host interactions have been highlighted. In the present study, we performed comprehensive miRNA profiling in EV71- and CA16-infected human umbilical vein endothelial cells (HUVECs) at multiple time points using high-throughput sequencing. The results showed that 135 known miRNAs exhibited remarkable differences in expression. Of these, 30 differentially expressed miRNAs presented opposite trends in EV71- and CA16-infected samples. Subsequently, we mainly focused on the 30 key differentially expressed miRNAs through further screening to predict targets. Gene ontology (GO) and pathway analysis of the predicted targets showed the enrichment of 14 biological processes, 9 molecular functions, 8 cellular components, and 85 pathways. The regulatory networks of these miRNAs with predicted targets, GOs, pathways, and co-expression genes were determined, suggesting that miRNAs display intricate regulatory mechanisms during the infection phase. Consequently, we ...Continue Reading

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Citations

Aug 15, 2018·Critical Reviews in Microbiology·Ilka EngelmannDidier Hober
Aug 16, 2019·Journal of Virology·Autumn C Holmes, Bert L Semler
Apr 25, 2020·International Journal of Molecular Sciences·Ruth Barral-ArcaAntonio Salas
Apr 4, 2021·International Journal of Molecular Sciences·Peiyu ZhuYuefei Jin

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Datasets Mentioned

BETA
EU812515.1
JN590244.1
GSE94551

Methods Mentioned

BETA
electrophoresis
miRNA-seq
Illumina sequencing
PCA

Software Mentioned

DAVID
_ toolkit
TargetScan
SPSS
fastx
PANTHER

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