Different neuron populations in the feline lateral cervical nucleus: a light and electron microscopic study with the retrograde axonal transport technique

The Journal of Comparative Neurology
R Flink, J Westman

Abstract

A comparative light and electron microscopic study was made of the two major neuron populations in the feline lateral cervical nucleus (LCN) that have an ascending projection--namely, the cervicothalamic and cervicomesencephalic neurons. The small population of neurons in the LCN that remained unlabelled after extensive injections into all known areas of LCN termination was also investigated. The intra-axonal retrograde tracing technique was employed and three different tracers were used: native horseradish peroxidase, horseradish peroxidase conjugated to wheat germ agglutinin, and cholera toxin subunit B (CTb). The diaminobenzidine and tetramethylbenzidine methods were used for the peroxidase reaction, and an immunocytochemical staining method with monoclonal antibodies was employed to localize CTb. The light microscopic cell count was performed on plastic-embedded semithin (2 micron) sections, and a section-embedding technique was used for sampling regions of interest for electron microscopy. The total number of neurons in the LCN was calculated to be 8,300, which is higher than numbers reported from other studies on frozen sections. It was concluded from control experiments that many neurons fail to counterstain in frozen se...Continue Reading

References

Feb 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M M Mesulam
Mar 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N K GonatasJ O Gonatas
Mar 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M M Mesulam, D L Rosene
Dec 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger
Jan 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D L Rosene, M M Mesulam
May 15, 1979·The Journal of Comparative Neurology·A D Craig, H Burton
Apr 2, 1979·Experimental Brain Research·J DestombesA Rouvière
Oct 15, 1978·The Journal of Comparative Neurology·A D Craig
Nov 1, 1978·Journal of Neurophysiology·A D Craig, D N Tapper
Jul 1, 1976·The Journal of Comparative Neurology·J A Rafols, C A Fox
Nov 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L Malmgren, Y Olsson
May 15, 1976·The Journal of Comparative Neurology·W M Falls, J S King
Jan 1, 1985·Progress in Neurobiology·G TrediciP Marmiroli
Sep 1, 1968·Brain Research·J Westman
Aug 25, 1972·Brain Research·I R Kaiserman-Abramof, A Peters
May 1, 1973·The Journal of Physiology·A G BrownH F Martin
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Oct 1, 1966·Quarterly Journal of Experimental Physiology and Cognate Medical Sciences·D F Horrobin
Mar 18, 1982·Brain Research·S K Itaya, G W van Hoesen
May 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K A Carson, M M Mesulam

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Citations

Jul 26, 1991·Brain Research·M V Smith, A V Apkarian
Jun 14, 2015·Experimental Eye Research·Andrea C NuschkeBalwantray C Chauhan
May 1, 1991·Cephalalgia : an International Journal of Headache·G A LambertJ W Lance
Mar 17, 2004·The Journal of Comparative Neurology·Leonora J MoutonGert Holstege
Feb 8, 1988·The Journal of Comparative Neurology·G J GieslerG Grant
Dec 21, 2007·The Journal of Comparative Neurology·Mengliang ZhangHans Hultborn
May 2, 2020·Chemical Communications : Chem Comm·Jessica L HaighW Bruce Turnbull
Mar 15, 1993·The Journal of Comparative Neurology·J Broman, B H Pubols
Aug 1, 1992·The Journal of Comparative Neurology·A D CraigA Blomqvist
Oct 1, 1987·The Journal of Comparative Neurology·M WibergA Blomqvist
Sep 1, 1987·The Journal of Comparative Neurology·G J GieslerJ T Katter
Oct 15, 1989·The Journal of Comparative Neurology·A V Apkarian, C J Hodge
May 15, 1989·The Journal of Comparative Neurology·J Broman, A Blomqvist
Aug 22, 1988·The Journal of Comparative Neurology·J Broman, J Westman

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