Different Organization of Type I Collagen Immobilized on Silanized and Nonsilanized Titanium Surfaces Affects Fibroblast Adhesion and Fibronectin Secretion

ACS Applied Materials & Interfaces
Nathalia Marín-ParejaMaria-Pau Ginebra

Abstract

Silanization has emerged in recent years as a way to obtain a stronger and more stable attachment of biomolecules to metallic substrates. However, its impact on protein conformation, a key aspect that influences cell response, has hardly been studied. In this work, we analyzed by atomic force microscopy (AFM) the distribution and conformation of type I collagen on plasma-treated surfaces before and after silanization. Subsequently, we investigated the effect of the different collagen conformations on fibroblasts adhesion and fibronectin secretion by immunofluorescence analyses. Two different organosilanes were used on plasma-treated titanium surfaces, either 3-chloropropyl-triethoxy-silane (CPTES) or 3-glycidyloxypropyl-triethoxy-silane (GPTES). The properties and amount of the adsorbed collagen were assessed by contact angle, X-ray photoelectron spectroscopy, optical waveguide lightmode spectroscopy, and AFM. AFM studies revealed different conformations of type I collagen depending on the silane employed. Collagen was organized in fibrillar networks over very hydrophilic (plasma treated titanium) or hydrophobic (silanized with CPTES) surfaces, the latter forming little globules with a beads-on-a-string appearance, whereas over...Continue Reading

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Citations

Aug 5, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nicholas G FischerConrado Aparicio
Dec 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Changcheng GaoBin Li
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yi ZhuJiandong Ding
Nov 4, 2020·The Journal of Cell Biology·María Benito-JardónMercedes Costell
Aug 29, 2021·Polymers·Ruth NaomiHasnah Bahari
Mar 1, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Abshar HasanLalit M Pandey

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