PMID: 8946214Oct 1, 1996Paper

Different pathways of the release of cytotoxic proteins in LAK cells

Immunology Letters
L P SashchenkoN V Gnuchev

Abstract

Human LAK cells were shown to release cytotoxic proteins by both Ca(2+)-dependent and Ca(2+)-independent mechanisms. CD3+ CD8+ CD16- and CD16+ CD8+ CD3- LAK cells were co-incubated with target cells in the presence of 4 mM EGTA. Although EGTA inhibited the exocytosis of cytolytic granules, supernatants obtained were cytotoxic for target cells. Cytotoxicity of CD3+ LAK cells and CD16+ LAK cells was due to cytotoxic proteins with MW 75 (p75), 35 (p35) and 22 (p22) kDa. LAK cells were also shown to release cytotoxic proteins by way of continuous secretion. After co-incubation in the absence of target cells LAK cells can secrete cytotoxic proteins with MW 75 (p75), 55 (p55), 38 (p38), 35 (p35), 25 (p25), 22 (p22) and 17 (p17) kDa.

References

Jan 1, 1977·Contemporary Topics in Immunobiology·P Golstein, E T Smith
Nov 1, 1977·The Journal of Experimental Medicine·A M Tartakoff, P Vassalli
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C C LiuJ D Young
Jul 14, 1989·Cell·G CarraraG P Tocchini-Valentini
Jan 1, 1986·Journal of Cellular Biochemistry·J D Young, Z A Cohn
Apr 1, 1988·Immunology Today·W R Clark
Aug 21, 1986·Nature·M S PasternackH N Eisen
Jan 1, 1986·Annual Review of Immunology·R B HerbermanJ R Ortaldo
Jan 1, 1993·The Journal of Experimental Medicine·E RouvierP Golstein
Jan 1, 1980·Scandinavian Journal of Immunology·S N BykovskajaA F Bykovsky

❮ Previous
Next ❯

Citations

Oct 31, 2003·The Journal of Biological Chemistry·Lidia P SashchenkoGeorgii P Georgiev

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.