May 27, 2014

Different profile of transcriptome between wheat Yunong 201 and its high-yield mutant Yunong 3114

BioRxiv : the Preprint Server for Biology
Feng ChenDangqun Cui

Abstract

Wheat is one of the most important crops in the world. With the exponentially increasing population and the need for ever increased food and feed production, an increased yield of wheat grain (as well as rice, maize and other grains) will be critical. Modern technologies are utilized to assist breeding programs. Such as the transcriptome sequencing, which greatly improves our genetic understanding, provides a platform for functional genomics research on crops. Herein, to get an overview of transcriptome characteristics of Yunong 3114, which is screened from the EMS mutagenized population of, a high quality Chinese winter noodle wheat, due to its different plant architecture as well as larger kernel size and higher grain weight, a high-throughput RNA sequencing based on next generation sequencing technology (Illumina) were performed. These unigenes were annotated by Blastx alignment against the NCBI non-redundant (nr), Clusters of orthologous groups (COG), gene orthology (GO), and the Kyoto Encyclopedia of Genesand Genomes (KEGG) databases. The 90.96% of the unigenes matched with protein in the NCBI nr database. Functional analysis identified that changes in several GO categories, including recognition of pollen, apoptotic proce...Continue Reading

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Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Biochemical Pathway
1,3-diiopanoate glyceryl ether
Size
Research
Rice (Dietary)
Grains
Crops, Agricultural
Genome
Genes

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