PMID: 9185163Jul 1, 1997Paper

Different response of cerebral and non-cerebral endothelial cells to cytotoxic hypoxia

Neurochemistry International
B AhlemeyerB Johannsen

Abstract

The present study investigated the effect of cytotoxic hypoxia on cerebral and non-cerebral endothelial cells. Hypoxia was induced by inhibiting the cellular respiratory chain with 1 mM sodium cyanide. Cerebral endothelial cells were damaged after 2 h of hypoxia as assessed by a decrease in cell viability by 25% and by a 2.7-fold higher lactate dehydrogenase release compared to controls. Additional glucose deprivation did not significantly exacerbate hypoxic injury. In addition, we found after 2 h of hypoxia an increase in the release of lactate of 1.02 and 0.42 mg/mg protein compared to 0.27 and 0.07 mg/mg protein in controls in the presence and absence of glucose, respectively. While the activity of ALP of cerebral endothelial cells was maintained at the control level, we found a significant decrease in the gamma-GT activity from 3.8 +/- 1.3 to 1.09 +/- 0.3 U/mg protein after 3 h of hypoxia in the presence as well as in the absence of glucose. The paracellular permeability of the cell monolayer decreased after 1 h and returned to control level after 3 h of hypoxia in the presence of glucose. Non-cerebral endothelial cells remained 98% viable with no change in the release of lactate dehydrogenase and lactate after 2 h of hypox...Continue Reading

References

Jan 1, 1987·Acta Neuropathologica·I Klatzo
Jan 1, 1994·Stroke; a Journal of Cerebral Circulation·Y OkadaG J del Zoppo
Feb 1, 1995·The Journal of Clinical Investigation·A V Tretyakov, H W Farber
Jul 1, 1994·Stroke; a Journal of Cerebral Circulation·D C HessK Buchanan
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