Different responses to mitogenic agents by Adult rat and human chromaffin cells in vitro

Endocrine Pathology
Arthur S Tischler, J C Riseberg

Abstract

Adrenal medullary hyperplasia and pheochromocytomas occur frequently in laboratory rats, both in the courseof aging and in response to prolonged administration of a variety of drugs and other substances. In contrast, these lesions are rare in humans. Rat chromaffin cells proliferate throughout life, but the proliferative capacities of human chromaffin cells are unknown. To determine whether the difference in prevalence of adrenal lesions might be correlated with differences in cell proliferation, adrenal medullary cells from 3 patients undergoing radical nephrectomy were maintained in vitro for up to 2 weeks in control medium or in the presence of nerve growth factor (NGF) and/or tetradecanoyl phorbol acetate (TPA), an activator of protein kinase C. Both NGF and TPA are known mitogens for neonatal and adult rat chromaffin cells. At intervals, the cultures were pulsed for up to 36 hours with bromodeoxyuridine (BrdU) to label S-phase nuclei. They were then fixed and consecutively stained for BrdU and for tyrosine hydroxylase, to confirm that labeled cells were chromaffin cells. Cells from adult female F344 rats were similarly maintained. Human chromaffin cells labeled with BrdU were extremely rare (less than 0.1 %) under all cult...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K UnsickerH Thoenen
Jul 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A S TischlerJ C Riseberg
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Jan 1, 1989·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A S TischlerR A DeLellis
Oct 17, 1985·Nature·L E Lillien, P Claude
May 15, 1968·Experientia·G MalvaldiM P Viola-Magni

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