Different roles of cAMP/PKA and PKC signaling in regulating progesterone and PGE2 levels in immortalized rat granulosa cell cultures

General and Comparative Endocrinology
Ala NemerDavid Ben-Menahem

Abstract

Follicular cells from various species secrete steroids and prostaglandins, which are crucial for reproduction, in response to gonadotropins. Here, we examined prostaglandin E2 (PGE2) secretion from immortalized rat granulosa cells derived from preovulaotry follicles expressing the rat follicle stimulating hormone receptor (denoted as FSHR cells) that produce progesterone in response to gonadotropins. The cells were stimulated with a) pregnant mare's serum gonadotropin (PMSG; a rat FSH receptor agonist), b) activators of the protein kinase A (PKA) pathway (forskolin and a cell permeable cAMP analog Dibutyryl-cAMP (DB-cAMP)) and c) protein kinase C (PKC) (12-O-tetradecanoylphorbol 13-acetate; TPA), alone and in combination for 24 h. Thereafter, PGE2 and progesterone levels in the culture media were determined. In accordance with previous studies, while PMSG and the PKA pathway activators induced progesterone accumulation in the media, TPA did not. In contrast, our data indicate that TPA, but neither PMSG, forskolin and DB-cAMP evoked PGE2 accumulation in the media. Western Blot analysis of cell lysate showed a drastic TPA induced increase of COX-2 levels, which was not seen with neither PMSG nor forskolin treatment. This associat...Continue Reading

Citations

May 30, 2019·Frontiers in Endocrinology·Livio Casarini, Pascale Crépieux
Oct 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Milena JankowskaMaria Stankiewicz
Mar 1, 2020·Cell and Tissue Research·Juan Manuel Teijeiro, Patricia Estela Marini
Jan 12, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jia ChenKamil Kuca

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