Different sealing materials preventing the microbial leakage into the screw-retained implant restorations: an in vitro analysis by DNA checkerboard hybridization

Clinical Oral Implants Research
Cássio do NascimentoVinícius Pedrazzi

Abstract

The aim of this controlled in vitro study was to identify and quantify up to 38 microbial species penetrating through the screw-retained implant prostheses with different sealing materials. Sixty morse cone implants were restored with single-unit screw-retained prostheses. All the components were randomly divided into five groups (n = 12) according to the proposed materials: (1) polytetrafluoroethylene tape+composite resin; (2) polytetrafluoroethylene tape+gutta-percha; (3) polytetrafluoroethylene tape+light-polymerized provisional composite; (4) cotton pellet+gutta-percha; and (5) cotton pellet+light-polymerized provisional composite. Human saliva was used as contaminant media, and DNA checkerboard hybridization was used to identify and quantify microbial species. Microbial leakage was observed in all groups: M. salivarium, S. pasteuri, P. nigrescens, and P. melaninogenica were the species presenting the highest values of genome count, prevalence, and proportion within the groups. The total microbial mean counts (×105 , ±SD) were as follows: Group 1 (2.81 ± 0.38), Group 2 (3.41 ± 0.38), Group 3 (6.02 ± 1.48), Group 4 (6.40 ± 1.42), and Group 5 (17.45 ± 1.67). Group 5 showed the higher microbial counts (P < 0.001). Moderate to ...Continue Reading

References

Apr 1, 1991·The Journal of Prosthetic Dentistry·E D AdrianK A Turner
Dec 1, 1994·Clinical Oral Implants Research·M QuirynenD van Steenberghe
Sep 1, 1993·Clinical Oral Implants Research·M Quirynen, D van Steenberghe
Mar 12, 1998·Journal of Clinical Periodontology·S S SocranskyR L Kent
Aug 1, 1998·Journal of Oral Rehabilitation·J S GuindyJ Meyer
Jan 12, 2005·The Journal of Oral Implantology·Ryan C TaylorEdwin A McGlumphy
Feb 22, 2005·Oral Microbiology and Immunology·J KatsoulisG R Persson
Mar 26, 2005·Journal of Dental Research·M QuirynenD van Steenberghe
Sep 13, 2006·Implant Dentistry·Ugo CovaniAntonio Barone
Jul 12, 2008·The Journal of Oral Implantology·Brandt WeiningerMike Beck
Aug 14, 2009·Clinical Implant Dentistry and Related Research·Jan CosynHugo De Bruyn
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jaan MännikCees Dekker
Feb 10, 2010·The Journal of Prosthetic Dentistry·Diane Yoshinobu TaricaSamantha T Truong
Mar 2, 2010·The Journal of Prosthetic Dentistry·Osvaldo D Moráguez, Urs C Belser
Jun 18, 2010·Journal of Clinical Microbiology·Cássio do NascimentoJuliana Aparecida Candido-Silva
Jan 5, 2011·Medicina oral, patología oral y cirugía bucal·Cristina Palma-CarrióMiguel Peñarrocha-Diago
May 3, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C do NascimentoR F de Albuquerque Junior
Nov 19, 2011·Clinical Oral Implants Research·Cássio do NascimentoRubens Ferreira de Albuquerque
Aug 29, 2012·Clinical Oral Implants Research·Koichi YamaneKiyoshi Koyano
Aug 29, 2012·Journal of Investigative and Clinical Dentistry·Salah SakkaMohammad Zakaria Nassani
Sep 5, 2012·The Journal of Prosthetic Dentistry·Sung-Do ParkHye-Won Cho
Oct 25, 2012·Clinical Oral Implants Research·Guido HeydeckeTim Joda
May 11, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sheila Pestana PassosMarco Antonio Bottino
Jul 11, 2013·Clinical Implant Dentistry and Related Research·Arthur Rodriguez Gonzalez CortesFaleh Tamimi
Mar 25, 2014·The International Journal of Oral & Maxillofacial Implants·Bjarni E PjeturssonIrena Sailer
Apr 1, 2014·International Journal of Oral and Maxillofacial Surgery·M C GoiatoE P Pellizzer
Apr 12, 2014·Clinical Oral Implants Research·Matteo AlbertiniPedro Bullón
Nov 18, 2014·Clinical Oral Implants Research·Long-Fei ZhuangNiklaus P Lang
Apr 16, 2015·Clinical Implant Dentistry and Related Research·Alexei Gama de Albuquerque CavalcantiFabiana Mantovani Gomes França

❮ Previous
Next ❯

Citations

Nov 22, 2016·The Journal of Oral Implantology·Todd R SchoenbaumRichard G Stevenson
Jan 7, 2020·The Journal of Oral Implantology·Cecília Alves de SousaWirley Gonçalves Assunção
Sep 9, 2017·British Dental Journal·A DawoodS Tanner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.