Different signatures of miR-16, miR-30b and miR-93 in exosomes from breast cancer and DCIS patients.

Scientific Reports
Qingtao NiHeidi Schwarzenbach

Abstract

Loading of microRNAs (miRNAs) into exosomes that are involved in cellular communication is a selective process. The current study investigates whether the enrichment of miRNAs in exosomes reflects the pathogenesis of breast cancer (BC) and ductal carcinoma in situ (DCIS). The levels of miRNAs were quantified in exosomes from plasma of 32 BC patients, 8 DCIS patients and 8 healthy women by TaqMan real-time PCR-based miRNA array cards containing 47 different miRNAs. Then, exosomal miR-16, miR-30b and miR-93 that displayed deregulation in the arrays were selected and analyzed in 111 BC patients, 42 DCIS patients and 39 healthy women by TaqMan real-time PCR. Identification of exosomes was performed by Western blot. The levels of exosomal miR-16 were higher in plasma of BC (p = 0.034) and DCIS (p = 0.047) patients than healthy women, and were associated with estrogen (p = 0.004) and progesterone (p = 0.008) receptor status. Particularly, in estrogen-positive patients miR-16 was significantly enriched in exosomes (p = 0.0001). Lower levels of exosomal miR-30b were associated with recurrence (p = 0.034). Exosomal miR-93 was upregulated in DCIS patients (p = 0.001). Our findings suggest that different signatures of miR-16, miR-30b and ...Continue Reading

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Citations

Oct 9, 2019·Expert Review of Molecular Diagnostics·Margaritis AvgerisAndreas Scorilas
Feb 5, 2019·Cancer Metastasis Reviews·Mariantonia LogozziStefano Fais
Aug 10, 2019·International Journal of Molecular Sciences·Qingqing LiuJianping Chen
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Methods Mentioned

BETA
biopsies
PCR
glycosylation
Precipitation
Protein Assay

Software Mentioned

SPSS
Thermo Fisher Scientific Analysis

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