PMID: 11920474Mar 29, 2002Paper

Different simian virus 40 genomic regions and sequences homologous with SV40 large T antigen in DNA of human brain and bone tumors and of leukocytes from blood donors

Cancer
Fernanda MartiniMauro Tognon

Abstract

Many studies found only a small fragment of the large T-antigen coding sequences in human tumors, raising doubts on authenticity of SV40 sequences detected in these samples. Five different regions of SV40 DNA were investigated in 106 fresh human tumor biopsies (25 brain, 69 bone, 12 Wilms' tumors), 71 tumor-derived cell cultures (38 from brain and 33 from bone tumors) and normal tissues (5 fresh bone biopsies and 38 buffy coats) by polymerase chain reaction (PCR) techniques and filter hybridization with specific oligoprobes. Expression of SV40 Tag sequences was analyzed in human tumor specimens by RT-PCR. SV40 large T-antigen sequences were detected at high prevalence, in human biopsies of primary brain (37-44%) and bone (21-37%) tumors, in cell cultures derived from brain (30-54%) and bone (53-80%) tumors. SV40 Tag sequences were detected in 29% of buffy coats of blood donors. However, only four brain tumor cell lines showed all the five regions of the SV40 genome investigated. Expression of SV40 Tag sequences was found in 11 of 27 (41%) human tumor samples. DNA sequence analysis indicated that the PCR-amplified products belong to the SV40 wild type. Polymerase chain reaction products of Tag middle portion from 20 of 78 (26%) ...Continue Reading

Citations

Jun 12, 2003·The American Journal of Medicine·Regis A VilchezJanet S Butel
Nov 9, 2006·Orphanet Journal of Rare Diseases·Katharina LeithnerHorst Olschewski
Jul 11, 2007·Infectious Agents and Cancer·Fernanda MartiniMauro Tognon
Dec 28, 2002·Journal of Virology·Riccardo DolcettiMauro Tognon
Apr 14, 2004·Journal of Virology·Sophie ShaikhKathleen Rundell
Jul 20, 2004·Clinical Microbiology Reviews·Regis A Vilchez, Janet S Butel
Feb 11, 2005·Molecular Medicine·Cristina MorelliMauro Tognon
Jul 21, 2005·Molecular Medicine·Valentina ParacchiniEmanuela Taioli
Dec 14, 2004·Cancer·Javier Hernández-LosaSantiago Ramón y Cajal
Feb 24, 2004·Journal of Cellular Physiology·Cristina MorelliMauro Tognon
Mar 17, 2012·Experimental Hematology·Elisa MazzoniMauro Tognon
May 18, 2004·International Journal of Cancer. Journal International Du Cancer·Mauro TognonGiuseppe Barbanti-Brodano
Oct 9, 2003·The Journal of Gene Medicine·Jianhua HuangHirofumi Hamada
Nov 30, 2006·International Journal of Cancer. Journal International Du Cancer·Keerti V Shah
Jan 16, 2008·Journal of Medical Primatology·E J VerschoorJ L Heeney
Sep 28, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Regis A Vilchez, Shimon Kusne
Mar 4, 2014·Seminars in Cell & Developmental Biology·Emily K ColvinAmanda L Hudson
Aug 23, 2012·PloS One·Manola ComarMauro Tognon

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