Abstract
A diverse antibody repertoire is primarily generated by the rearrangement of V, D, and J genes and subsequent somatic hypermutation (SHM). Class-switch recombination (CSR) produces various isotypes and subclasses with different functional properties. Although antibody isotypes and subclasses are considered to be produced by both direct and sequential CSR, it is still not fully understood how SHMs accumulate during the process in which antibody subclasses are generated. Here, we developed a new next-generation sequencing (NGS)-based antibody repertoire analysis capable of identifying all antibody isotype and subclass genes and used it to examine the peripheral blood mononuclear cells of 12 healthy individuals. Using a total of 5,480,040 sequences, we compared percentage frequency of variable (V), junctional (J) sequence, and a combination of V and J, diversity, length, and amino acid compositions of CDR3, SHM, and shared clones in the IgM, IgD, IgG3, IgG1, IgG2, IgG4, IgA1, IgE, and IgA2 genes. The usage and diversity were similar among the immunoglobulin (Ig) subclasses. Clonally related sequences sharing identical V, D, J, and CDR3 amino acid sequences were frequently found within multiple Ig subclasses, especially between IgG...Continue Reading
References
Mar 1, 1992·The Journal of Experimental Medicine·T DefranceJ Banchereau
Jul 1, 1992·European Journal of Immunology·G SiebenkottenA Radbruch
Jul 13, 1990·Cell·M MatsuokaH Sakano
Apr 22, 1988·Cell·S LutzkerF W Alt
Sep 1, 1988·The Journal of Experimental Medicine·D A Lebman, R L Coffman
Apr 1, 1985·The Journal of Experimental Medicine·S H ClarkeM Weigert
Aug 1, 1994·Immunology Today·B Chang, P Casali
Feb 1, 1994·The Journal of Experimental Medicine·F BrièreJ Banchereau
Mar 1, 1996·The Journal of Experimental Medicine·F MalisanH Martinez-Valdez
Jun 16, 1999·Journal of Molecular Evolution·L G CowellT B Kepler
Jun 20, 2001·Immunology·K RosnerP J Gearhart
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·K Kinoshita, T Honjo
Sep 11, 2001·Experimental and Clinical Immunogenetics·M P Lefranc
May 25, 2004·Infection and Immunity·Jianhui ZhouDonald C Reason
Jun 11, 2004·Immunity·Tasuku HonjoSidonia Fagarasan
Oct 4, 2006·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Isabelle MeytsBoeck De
Dec 13, 2006·Molecular Immunology·Takaji MatsutaniTsunetoshi Itoh
Mar 17, 2007·Annals of Clinical Biochemistry·Amir H Abdul Latiff, Michael A Kerr
Jun 16, 2007·Immunity·Bing HeAndrea Cerutti
Nov 20, 2008·Blood·Pierre BruhnsMarc Daëron
Sep 5, 2009·Science·Laura M WalkerDennis R Burton
Dec 4, 2010·Molecular Immunology·Takaji MatsutaniTsunetoshi Itoh
Jan 18, 2012·The Journal of Experimental Medicine·Huizhong XiongJuan J Lafaille
Feb 10, 2012·The New England Journal of Medicine·John H StoneVikram Deshpande
Jul 17, 2013·The Journal of Experimental Medicine·Yang LiScott E Hensley
Aug 21, 2013·Frontiers in Immunology·Andrew M Collins, Katherine J L Jackson
Jun 11, 2014·Immunology and Cell Biology·Katherine J L JacksonAndrew M Collins
Jun 18, 2014·Immunology and Cell Biology·Menno C van Zelm
Nov 5, 2014·Frontiers in Immunology·Gestur VidarssonTheo Rispens
Dec 3, 2014·Virology·Christoph C CarterDavey M Smith
Dec 8, 2014·Lancet·Terumi KamisawaJohn H Stone
Apr 12, 2015·Blood·Michael A PulsipherStephan A Grupp
Mar 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Samiran MondalTasuku Honjo
Aug 3, 2016·ELife·Felix HornsStephen R Quake
Oct 13, 2016·BMC Immunology·Kazutaka KitauraRyuji Suzuki
Citations
Apr 10, 2018·PLoS Pathogens·Simone I RichardsonLynn Morris
Jan 26, 2018·European Journal of Immunology·Lisa K BlumWilliam H Robinson
Jun 27, 2018·Immunological Reviews·Deborah Dunn-WaltersAlex Stewart
Dec 18, 2018·PloS One·Yasunari NakasoneRyuji Suzuki
Feb 12, 2019·Nature Genetics·Xihao HuX Shirley Liu
Apr 5, 2019·Human Vaccines & Immunotherapeutics·Akio MiyasakaYasuhiro Takikawa
Apr 12, 2019·Current Opinion in HIV and AIDS·Lela Kardava, Susan Moir
Sep 8, 2019·Journal of Biomedical Science·Ricardo Dante LardoneGustavo Alejandro Nores
May 9, 2019·Life Science Alliance·Shinya HatanoYasunobu Yoshikai
Jul 28, 2020·International Journal of Molecular Sciences·Rodolfo BianchiniErika Jensen-Jarolim
Aug 13, 2020·JGH Open : an Open Access Journal of Gastroenterology and Hepatology·Yoichi KakutaAtsushi Masamune
Sep 25, 2018·Frontiers in Immunology·Line Ohm-LaursenSteven H Kleinstein
Jun 16, 2019·Scientific Reports·Makoto HirokawaRyuji Suzuki
Jun 11, 2020·International Journal of Molecular Sciences·Hiroaki ShigematsuRyuji Suzuki
Jul 9, 2020·Frontiers in Immunology·Fumi Sato-KanekoTomoko Hayashi
Aug 1, 2019·Archivum Immunologiae Et Therapiae Experimentalis·Amrita Khokar, Sudhir Gupta
Mar 30, 2018·Frontiers in Immunology·Neha Chaudhary, Duane R Wesemann
Jul 25, 2019·Frontiers in Immunology·Marvyn T KoningHendrik Veelken
Oct 24, 2020·The Journal of Experimental Medicine·Ida LindemanLudvig M Sollid
Jan 10, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Ioannis PeppasMieke Van Hemelrijck
Jan 29, 2021·Cancer Medicine·Yasufumi SatoKenzo Shimazu
Feb 2, 2021·Frontiers in Microbiology·Binbin HongTianlei Ying
Nov 3, 2020·MAbs·Luke BurmanDavid J King
Oct 26, 2020·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Hiroki YamaguchiIchiei Narita
Feb 20, 2021·MAbs·Thach H ChuMargaret E Ackerman
Mar 23, 2021·Cancer Immunology, Immunotherapy : CII·Yoshiro NakaharaTetsuro Sasada
Mar 30, 2021·Lupus·Ibrahim AlmaghlouthMurray Urowitz
Feb 13, 2019·Trends in Immunology·Timon DamelangAmy W Chung
Apr 2, 2021·Brain, Behavior, and Immunity·Wakiro SatoTakashi Yamamura
Jul 10, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Binbin HongLizhi Wang
Aug 17, 2021·Heliyon·Yohei FunakoshiHironobu Minami
Aug 24, 2021·Annals of Neurology·Kimitoshi KimuraTakashi Yamamura
Oct 12, 2021·British Journal of Haematology·Kisato NosakaTakashi Ishida
Oct 19, 2021·ELife·Marie GhraichyJohannes Trück
Nov 13, 2021·Frontiers in Immunology·Xiujia YangZhenhai Zhang