Different Stationary Phase Selectivities and Morphologies for Intact Protein Separations

Chromatographia
A AstefaneiM Camenzuli

Abstract

The central dogma of biology proposed that one gene encodes for one protein. We now know that this does not reflect reality. The human body has approximately 20,000 protein-encoding genes; each of these genes can encode more than one protein. Proteins expressed from a single gene can vary in terms of their post-translational modifications, which often regulate their function within the body. Understanding the proteins within our bodies is a key step in understanding the cause, and perhaps the solution, to disease. This is one of the application areas of proteomics, which is defined as the study of all proteins expressed within an organism at a given point in time. The human proteome is incredibly complex. The complexity of biological samples requires a combination of technologies to achieve high resolution and high sensitivity analysis. Despite the significant advances in mass spectrometry, separation techniques are still essential in this field. Liquid chromatography is an indispensable tool by which low-abundant proteins in complex samples can be enriched and separated. However, advances in chromatography are not as readily adapted in proteomics compared to advances in mass spectrometry. Biologists in this field still favour ...Continue Reading

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Citations

Jul 2, 2019·Biomedical Chromatography : BMC·Lijuan KangWenying Jian
Nov 2, 2019·Journal of Pharmaceutical and Biomedical Analysis·Julien CamperiNathalie Delaunay
May 14, 2021·Journal of the American Society for Mass Spectrometry·Jake A MelbyYing Ge
Dec 21, 2017·Analytical Chemistry·ZhiYu LiFred E Regnier
Mar 30, 2019·Analytical Chemistry·David J ClarkHui Zhang

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Methods Mentioned

BETA
ubiquitination
reversed phase chromatography
size exclusion chromatography
glycosylation
acetylation
immunodepletion
a size exclusion
SMA
flow chromatography

Software Mentioned

IMAC
Comsol

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