Different structural requirements for functional ion pore transplantation suggest different gating mechanisms of NMDA and kainate receptors

Journal of Neurochemistry
Carmen VillmannMichael Hollmann

Abstract

Although considerable progress has been made in characterizing the physiological function of the high-affinity kainate (KA) receptor subunits KA1 and KA2, no homomeric ion channel function has been shown. An ion channel transplantation approach was employed in this study to directly test if homomerically expressed KA1 and KA2 pore domains are capable of conducting currents. Transplantation of the ion pore of KA1 or KA2 into GluR6 generated perfectly functional ion channels that allowed characterization of those electrophysiological and pharmacological properties that are determined exclusively by the ion pore of KA1 or KA2. This demonstrates for the first time that KA1 and KA2 ion pore domains are intrinsically capable of conducting ions even in homomeric pore assemblies. NMDA receptors, similar to KA1- or KA2-containing receptors, function only as heteromeric complexes. They are composed of NR1 and NR2 subunits, which both are non-functional when expressed homomerically. In contrast to NR1, the homomeric NR2B ion pore failed to translate ligand binding into pore opening when transplanted into GluR6. Similarly, heteromeric coexpression of the ion channel domains of both NR1 and NR2 inserted into GluR6 failed to produce function...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A T EldefrawiP N Usherwood
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Egebjerg, S F Heinemann
Jan 1, 1994·Annual Review of Neuroscience·M Hollmann, S Heinemann
May 23, 1998·The Journal of Physiology·R Bähring, M L Mayer
Jan 10, 2002·Neuron·Alexander I SobolevskyLonnie P Wollmuth
Jun 7, 2002·Current Opinion in Neurobiology·Dzwokai Ma, Lily Yeh Jan
Apr 15, 2003·The Journal of Physiology·Claudia JatzkeLonnie P Wollmuth
Jun 5, 2003·Nature Reviews. Neuroscience·Juan Lerma
Aug 22, 2003·The Journal of Biological Chemistry·Nathalie Strutz-SeebohmMichael Hollmann
Jan 15, 2004·Molecular Pharmacology·Nansheng ChenLynn A Raymond
Feb 10, 2004·Neuron·Alexander I SobolevskyLonnie P Wollmuth
Feb 24, 2004·Annual Review of Physiology·Mark L Mayer, Neali Armstrong
May 29, 2004·Trends in Neurosciences·Lonnie P Wollmuth, Alexander I Sobolevsky
Dec 8, 2004·The Journal of Biological Chemistry·Lokanatha ValluruGeoffrey T Swanson
Apr 26, 2005·Neuron·Christopher J Hatton, Pierre Paoletti
Jun 23, 2005·The Journal of Biological Chemistry·Hongjie YuanStephen F Traynelis
Nov 11, 2005·Nature·Hiroyasu FurukawaEric Gouaux
Dec 16, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Arnaud RuizChristophe Mulle
Jun 30, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yukiko Nasu-NishimuraKatherine W Roche
Jul 25, 2006·Cell and Tissue Research·Georg Köhr
Oct 3, 2006·Molecular and Cellular Neurosciences·Jutta HoffmannMichael Hollmann
Oct 21, 2006·Biochemical Society Transactions·F Coussen, C Mulle
May 17, 2007·The Journal of General Physiology·Alexander I SobolevskyLonnie P Wollmuth
Jul 17, 2007·Trends in Neurosciences·Ingo H GregerAndrew C Penn
Jan 8, 2008·Journal of Molecular Biology·Carsten Schmidt, Michael Hollmann

❮ Previous
Next ❯

Citations

Sep 22, 2012·The Journal of Biological Chemistry·Bea UntererCarmen Villmann
Feb 25, 2014·Nature Communications·Timothy J WildingJames E Huettner
Sep 21, 2010·Trends in Pharmacological Sciences·David PerraisChristophe Mulle
Apr 18, 2012·Cell Reports·Andreas ReinerEhud Y Isacoff

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Yukiko Nasu-NishimuraKatherine W Roche
Biochemical and Biophysical Research Communications
Dayna M HayesKatherine W Roche
Proceedings of the National Academy of Sciences of the United States of America
Sabine M SchmidMichael Hollmann
Trends in Pharmacological Sciences
Frédéric JaskolskiChristophe Mulle
© 2022 Meta ULC. All rights reserved