Different susceptibility of B19 virus and mice minute virus to low pH treatment

Transfusion
Nicola BoschettiJohannes Blümel

Abstract

Parvoviridae are small nonenveloped viruses that are known to be highly resistant to physico-chemical treatments. Because low pH is frequently applied to process intermediates or final products, the impact of such conditions on the human erythrovirus B19 (B19V) and the mouse parvovirus (mice minute virus, MMV) was assessed, which is often used as a model for B19V. Owing to the lack of a suitable cultivation and/or detection system for B19V no such data exist so far. Virus inactivation was monitored by decrease of infectivity and loss of capsid integrity. Infectious B19V was quantified by detection of virus-specific messenger RNA from Ku812Ep6 cells. To measure capsid integrity, endonucleases were added after exposure to low pH and the encapsidated (endonuclease-protected) virus DNA was quantified by real-time PCR. B19V was inactivated greater than 5 log after 2 hours at pH 4, whereas MMV was resistant over 9 hours. Infectivity data strongly correlated with data obtained by the endonuclease assay. Capsid disintegration was observed in immunoglobulin G as well as in different albumin solutions. Temperature and pH showed concerted impact on B19V capsid disintegration. Our data show that B19V is much more vulnerable toward low pH c...Continue Reading

References

Nov 1, 1987·The Journal of General Virology·J MoriJ P Clewley
Oct 1, 1986·Journal of Clinical Microbiology·L J AndersonP P Mortimer
Aug 1, 1987·Journal of Virology·K OzawaN Young
Jul 16, 1999·Journal of Clinical Microbiology·Q T NguyenA Garbarg-Chenon
Jan 29, 2000·British Journal of Haematology·M HinoN Tatsumi
Jul 4, 2001·Psychiatric Services : a Journal of the American Psychiatric Association·UNKNOWN Committe on Psychiatry and the Community of the Group for the Advancement of Psychiatry
Jul 5, 2002·Clinical Microbiology Reviews·Erik D Heegaard, Kevin E Brown
Jul 16, 2002·Pathologie-biologie·Ruth Laub, Paul Strengers
Aug 21, 2002·Journal of Virology·Annabelle ServantAntoine Garbarg-Chenon
Oct 9, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Hartwig W LehmannSusanne Modrow
Oct 19, 2002·Transfusion·Johannes BlümelJohannes Löwer
Nov 8, 2002·Transfusion·Johannes BlümelAnna Maria Eis-Hübinger
Nov 21, 2002·Virology·Kati HokynarKlaus Hedman
Feb 5, 2003·International Journal of Clinical Pharmacology and Therapeutics·S S JoshiA D Halpner
Apr 3, 2003·Vox Sanguinis·M YunokiK Ikuta
May 13, 2003·Reviews in Medical Virology·Faraj BarahJonathan R Kerr
Aug 26, 2003·Biologicals : Journal of the International Association of Biological Standardization·Nicola BoschettiChristoph Kempf

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Citations

Oct 29, 2005·Journal of Virology·Johannes BlümelJohannes Löwer
Apr 15, 2014·Thrombosis Research·Albrecht Gröner
Mar 31, 2010·Biologicals : Journal of the International Association of Biological Standardization·Santiago CaballeroJuan I Jorquera
Apr 15, 2008·Trends in Microbiology·Carole E HarbisonColin R Parrish
Mar 14, 2008·Biologicals : Journal of the International Association of Biological Standardization·M StuckiC Kempf
Sep 27, 2005·Transfusion·Grigori G Prikhod'ko
Dec 20, 2005·Vox Sanguinis·F G TerpstraF A C van Engelenburg
Aug 8, 2007·Transfusion Medicine·A Parsyan, D Candotti
Dec 12, 2007·Vox Sanguinis·J F FryerS A Baylis
Mar 15, 2012·Transfusion·Albrecht GrönerWolfram Schäfer
Feb 22, 2012·Transfusion·Johannes BlümelSally A Baylis
Sep 17, 2013·Transfusion·Sally A BaylisJohannes Blümel
Apr 4, 2006·Biologicals : Journal of the International Association of Biological Standardization·Christoph KempfNicola Boschetti
Feb 17, 2006·Molecular Aspects of Medicine·F WatzingerT Lion
Jul 31, 2016·Biologicals : Journal of the International Association of Biological Standardization·Raymond W Nims, S Steve Zhou
Nov 18, 2017·Transfusion·Albrecht GrönerNathan J Roth
Feb 26, 2021·Clinical and Experimental Vaccine Research·Rocío Silvia Sandoval-MonzónLuis Felipe Ruiz-García

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