PMID: 3117103Jul 14, 1987Paper

Different types of microheterogeneity of human thyroxine-binding prealbumin

Biochemistry
Tom M PetterssonHans Jörnvall

Abstract

Human thyroxine-binding prealbumin or transthyretin has been isolated by three different methods. In all cases, the isolated material is microheterogeneous in an isoelectric focusing system, revealing a pattern of at least 10 bands. These subforms represent tetramers. Dissociation of the protein in serum samples and dissociation in the presence of urea or sodium dodecyl sulfate (SDS) reveal eight other forms, differing in isoelectric point and concluded to be monomers. Two different sets of dissociated forms are identified, one from urea treatment and the other from SDS treatment. The latter set is apparently also present in serum. Interpretations are complicated by multiplicities of all forms, atypical electrophoretic migrations, nonidentical effects of urea and SDS, and the absence of dimer under most conditions. However, reassociations of monomers and formation of interspecies hybrid molecules identify dimers and clarify interpretations of the dissociated sets. In at least five of the forms likely to represent monomers, the difference is dependent on the nature of the SH group at Cys-10, which can be oxidized, can be reduced, or can be in a mixed disulfide, probably with glutathione and its degradation products. Amino acid s...Continue Reading

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Citations

Feb 1, 1991·Journal of Protein Chemistry·R KaiserHans Jörnvall
Apr 23, 2005·Cell and Tissue Research·H A MontecinosE M Rodríguez
Jun 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G SchreiberS C Nicol
Nov 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Essam RefaiLisa Juntti-Berggren
Sep 4, 2009·The FEBS Journal·P Prapunpoj, L Leelawatwattana
Oct 15, 2014·Journal of Cellular and Molecular Medicine·Andrea HenzeAysel Ozpinar
Aug 9, 2016·PloS One·Elżbieta WieczorekAndrzej Ożyhar
Jan 16, 1989·Biochemical and Biophysical Research Communications·Tom M PetterssonHans Jörnvall
Nov 6, 2014·Biomolecular Concepts·Michael LandrehHans Jörnvall
Jul 1, 1992·Clinical Genetics·I L AlvesM J Saraiva

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