Differential abilities of chicken Pit1 isoforms to regulate the GH promoter: evidence for synergistic activation

Endocrinology
Malini Mukherjee, Tom E Porter

Abstract

Pit1, pituitary-specific transcription factor 1, regulates differentiation of cells of the Pit1 lineage in the anterior pituitary and the synthesis of peptide hormones by these cell types, including GH. Pit1 is characterized by an N-terminal transactivation domain and a C-terminal POU domain. Alternative forms of Pit1, differing from each other in the N-terminal domain, have been reported in several species, but the functional implication of having multiple isoforms is not known. Several PIT1 mRNA transcripts exist in chickens that have not been characterized. This study was conducted to determine which, if any, of the chicken Pit1 isoforms regulate the chicken GH (cGH) promoter. During the course of this work, Pit1β2, a novel isoform of chicken Pit1, was discovered. Effects of known and novel isoforms (Pit1α, Pit1β1, Pit1β2, and Pit1γ) on cGH promoter activity were characterized in chicken Leghorn male hepatoma cells. Three of the isoforms, Pit1α, Pit1β1, and Pit1β2, activated the cGH promoter, whereas Pit1γ did not. Results from gel-shift assays indicated that Pit1γ does not bind to the proximal Pit1-bindng site of the cGH promoter, suggesting a possible mechanism underlying its inactivity. We found a functional advantage for...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·A E MorrisL A Chasin
Nov 15, 1996·The Journal of Biological Chemistry·S E Diamond, A Gutierrez-Hartmann
Jan 10, 2001·Canadian Journal of Physiology and Pharmacology·S HarveyK L Hull
Jul 2, 2004·Experimental Biology and Medicine·Stephen C Y IpFrederick C Leung
Jun 28, 2011·General and Comparative Endocrinology·Daisuke MuraseTakeshi Ohkubo
Dec 14, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kristina Heuck-KnubelTom E Porter

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Citations

Aug 28, 2019·General and Comparative Endocrinology·Ellyse B NoyBert De Groef
Mar 15, 2021·Environmental Science and Pollution Research International·Hebatallah Abdel MoniemGuohong Chen

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